Bacterial Motility- Flagellao Eukaryotes Moves side to side Microtubules o Prokaryotes Moves like propeller Motor proteins o Staining Mordant on flagella Carbolfuchsin (pink) simple stain OR fluorescent antibodies - Structure of Flagella (Bacteria)o Filament Hollow, rigid, cylindrical Composed of flagellin Some have sheaths o Hook Connects filament -> cello Basal Body Anchors flagella -> cell wall rings power the motor- Movement of Flagella (Bacteria)o Runs Counterclockwise rotation Forward motiono Tumbles Clockwise rotation Disrupts run - Chemotaxis o Taxis = movement toward or away from stimulus Lower frequency of tumbles Directed by chemoreceptors on cell surface o Biased Random Walk- Spirocheteso Mobile via axial filaments Endoflagella Anchored at one end of cell The whole cell moves when it rotates, causing a corkscrew motion- Fimbriae and Pilio Similar in structure to flagella, but shorter and thinnero Fimbriae Helps with attachment No motiono Pili Transfer of DNA from cell to cell Twitching and gliding
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