BCHM 4116 1st Edition Lecture 25 Outline of Last Lecture I Regulation of Transcription and Regulation of Gene Expression Cont II Gene Transcription in Eukaryotes Outline of Current Lecture I The structure and function of RNA polymerase II the mRNA synthesizing RNA polymerase II Transcription regulation in eukaryotes including Current Lecture Chapter 29 Transcription and Regulation of Gene Expression cont 2 a The structure and function of RNA polymerase II the mRNA synthesizing RNA polymerase i CTD is essential to RNA pol II function ii Only RNA pol II whose CTD is not phosphorylated can initiate transcription 1 But phosphorylation transcription elongation 2 Phosphorylation triggers conversion of an initiation complex into an elongation complex b Transcription regulation in eukaryotes including i General features of gene regulatory sequences promoters enhancers and response elements 1 Promoters consist of 2 features a Core element near transcription start site where general transcription factors bind b Regulatory elements 2 Enhancers assist initiation a Location of enhancers relative to transcription start site is not fixed i May be thousand nucleotides away from promoter ii Act to enhance transcription even if positioned downstream b BIDIRECTIONAL in that they function in either orientation c promiscuous b c they stimulate transcription from an promoter that happens to be in their vicinity These notes represent a detailed interpretation of the professor s lecture GradeBuddy is best used as a supplement to your own notes not as a substitute d enhancer function is dependent on recognition by a specific transcription factor 3 Response Elements promoter responsive to common regulation a Ex heat shock element HSE b Glucocorticoid response element GRE c Found in promoter region
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