SC CHEM 333 - Thin Layer Chromatography of Analgesics (TLC) Pre-Lab (7 pages)

Previewing pages 1, 2 of 7 page document View the full content.
View Full Document

Thin Layer Chromatography of Analgesics (TLC) Pre-Lab



Previewing pages 1, 2 of actual document.

View the full content.
View Full Document

Unformatted text preview:

Organic Chemistry 333L section 03M Laboratory Pre Lab Thin Layer Chromatography of Analgesics TLC By Cameron Kahn 14 January 2019 TA James Collie 1 Page Objective In this experiment one will learn how to set up the apparatus and perform a thin layer chromatography analysis on various standard analgesics The experiment will also teach one to calculate the Rf value and determine the identity of an unknown analgesic by comparing its Rf value to those of the standard analgesics CITATION Geo121 l 1033 By doing the experiment one will learn to identify the content of over the counter analgesics Theory TLC is a simple quick and inexpensive procedure that gives the chemist a quick answer as to how many components are in a mixture TLC is also used to support the identity of a compound in a mixture when the Rf of a compound is compared with the Rf of a known compound preferably both run on the same TLC plate A TLC plate is a sheet of glass metal or plastic which is coated with a thin layer of a solid adsorbent usually silica or alumina A small amount of the mixture to be analyzed is spotted near the bottom of this plate The TLC plate is then placed in a shallow pool of a solvent in a developing chamber so that only the very bottom of the plate is in the liquid This liquid or the eluent is the mobile phase and it slowly rises up the TLC plate by capillary action As the solvent moves past the spot that was applied equilibrium is established for each component of the mixture between the molecules of that component which are adsorbed on the solid and the molecules which are in solution In principle the components will differ in solubility and in the strength of their adsorption to the adsorbent and some components will be carried farther up the plate than others When the solvent has reached the top of the plate the plate is removed from the developing chamber dried and the separated components of the mixture are visualized If the compounds are colored visualization is



View Full Document

Access the best Study Guides, Lecture Notes and Practice Exams

Loading Unlocking...
Login

Join to view Thin Layer Chromatography of Analgesics (TLC) Pre-Lab and access 3M+ class-specific study document.

or
We will never post anything without your permission.
Don't have an account?
Sign Up

Join to view Thin Layer Chromatography of Analgesics (TLC) Pre-Lab and access 3M+ class-specific study document.

or

By creating an account you agree to our Privacy Policy and Terms Of Use

Already a member?