SC CHEM 333 - Thin Layer Chromatography of Analgesics (TLC) Post-Lab (4 pages)

Previewing page 1 of 4 page document View the full content.
View Full Document

Thin Layer Chromatography of Analgesics (TLC) Post-Lab



Previewing page 1 of actual document.

View the full content.
View Full Document

Unformatted text preview:

Organic Chemistry 333L section 03M Laboratory Post Lab Thin Layer Chromatography of Analgesics TLC By Cameron Kahn 8 October 2013 TA James Collie 1 Page Results The thin layer of chromatography paper TLC was measured to be a length of 9cm from top to bottom The solvent front was measured to be 7 0cm from bottom to the point at which it stopped moving up The baseline was measured to be 1cm from the bottom of the paper The four analgesics used in this experiment were Aspirin acetylsalicylic acid Acetaminophen Ibuprofen caffeine and the unknown A sample The two phases in this experiment were stationary adsorbent which was silica gel and mobile phase solvent which was comprised of 95 ethyl acetate and 5 acetic acid Handy The mobile phase of Aspirin was 5 75cm with an Rf value of 0 821 The mobile phase of Acetaminophen was measured at 4 50cm with the Rf value of 0 643 The mobile phase of Ibuprofen was 1 40cm with an Rf value of 0 20 The mobile phase of caffeine was 6 50cm with an Rf value of 0 929 Lastly the mobile phase of sample unknown A was measured to be 4 40cm with an Rf value of 0 629 From comparing the Rf values of each solutions being tested the unknown sample had similar Rf value of Aspirin Observations The TLC plate had a white front with a clear plastic backing The adsorbent on the TLC plate was the white front which was presumed to be either silica gel or alumina on the basis that the two are the most commonly used adsorbents on any TLC plates UC Boulder The solvent used in the experiment was clear The initial colors noticed from each drop of solution on the TLC paper were as follows Aspirin was clear Acetaminophen was a faint light yellow hue Ibuprofen was clear with no noticeable color Caffeine was white in color and Unknown A was a faint light yellow A strong odor from the ether was noticed with no smell coming from the TLC paper While performing the experiment one could see the TLC place become moist but he she could not see the compounds Once the TLC



View Full Document

Access the best Study Guides, Lecture Notes and Practice Exams

Loading Unlocking...
Login

Join to view Thin Layer Chromatography of Analgesics (TLC) Post-Lab and access 3M+ class-specific study document.

or
We will never post anything without your permission.
Don't have an account?
Sign Up

Join to view Thin Layer Chromatography of Analgesics (TLC) Post-Lab and access 3M+ class-specific study document.

or

By creating an account you agree to our Privacy Policy and Terms Of Use

Already a member?