Introduction/Microscope Intro/Cell Basics/Cell Division A215 LabPowerPoint Introductions • PDFs of intros can be downloaded from the Lab website’s “Lab Resources” page: http://www.indiana.edu/~anat215/lab/resources.htmWhat is Histology? • Histology=Microscopic Anatomy – Learn what makes up the different tissues in the body – Learn how different tissues relate to structure and function (or malfunction)Types of Microscopy • Electron Microscopy – Powerful magnifying technique used to visualize intracellular structures – Up to 105xMagnification Demo (Pin Head) x33Magnification Demo (Pin Head) x100Magnification Demo (Pin Head) x250Magnification Demo (Pin Head) x1000Magnification Demo (Pin Head) x5000Magnification Demo (Pin Head) x30,000Types of Microscopy • Electron Microscopy – Powerful magnifying technique used to visualize intracellular structures – Up to 105x • Light Microscopy – Thin section of preserved tissue is cut and placed on a slide and stained – 4x-1000x • in A215, however, you will magnify at most about 400x (40x magnification of virtual microscope objective lens x 10x lens in eyepiece) • You need to multiply the number shown on the VM by 10 to find the actual magnificationTypes of sectioning Cross Section Longitudinal ObliqueMuscle Section? Section? Section?Cell Basics Cell Membrane Nucleus Cytoplasm NucleolusCell Basics • Not all cells look the same • Cells can vary in terms of visible organelles and shapePDFs of A215 lab electron micrographs can be downloaded using the link at the bottom of the “Lab Resources” webpage Cell OrganellesRough Endoplasmic ReticulumCell OrganellesMitochondria" Centrioles " 9 sets of tripletsMicrovilliCilia Type of sectioning? Section? Section?Cilia 9 doublets +2Cell Division Prophase “Prepare” Metaphase “Meet” Anaphase “Apart” Telophase “Two”Donor Introduction • Donors are people who freely donated their bodies for education • Male and Female Donors (no longer referred to here as cadavers) • New donors re-introduced every Fall • Prosection done by medical students • Remains are cremated and returned to families • PLEASE SHOW THEM APPROPRIATE RESPECTWhat phase? What phase? What phase? What