UNC-Chapel Hill ENVR 132 - DNA Damage and Repair (13 pages)

Previewing pages 1, 2, 3, 4 of 13 page document View the full content.
View Full Document

DNA Damage and Repair



Previewing pages 1, 2, 3, 4 of actual document.

View the full content.
View Full Document
View Full Document

DNA Damage and Repair

82 views

Lecture Notes


Pages:
13
School:
University of North Carolina at Chapel Hill
Course:
Envr 132 - Biochemical and Molecular Toxicology
Biochemical and Molecular Toxicology Documents

Unformatted text preview:

DNA damage and repair William Kaufmann 31 325 LCCC 966 8209 william kaufmann pathology unc edu 1 The four R s of human DNA metabolism 2 Mechanism of cytotoxicity of topoI poisons 3 TopoII reactions DNA strand scission by topoII poisons Sites of alkylation of DNA 4 BP adduct on DNA An arrested replicon 5 Pathways of DNA repair O6 alkylguanine DNA alkyltransferase suicide protein transfers methyl group Base excision repair glycosylase AP endonuclease DNA polymerase ligase Nucleotide excision repair transcription coupled and global Cross link repair Fanconi s anemia genes and BRCA2 Double strand break repair Homologous recombination and non homologous end joining Heteroduplex and mismatch repair Post replication repair translesion synthesis by pol eta zeta iota or kappa Nucleotide excision repair 6 Pathways of repair of DNA double strand breaks Non homologous end joining DNA PK Ku70 80 XRCC1 DNA ligase IV immunoglobulin and TCR gene rearrangement Homologous recombination rad51 52 XRCC2 3 4 7 Crosslink repair requires Fanconi s anemia genes and BRCA2 Familial cancer syndromes Ataxia telangiectasia cell cycle checkpoint function DNA repair ATM NBS1 MRE 11 Fanconi s anemia DNA repair BRCA2 and 5 other genes HNPCC mismatch repair hMSH2 hMLH1 PMS2 hMSH6 Xeroderma pigmentosum nucleotide excision repair and post replication repair 8 XP genes Familial breast cancer I S and G2 checkpoint responses BRCA1 Li Fraumeni syndrome cell cycle checkpoint function and DNA repair P53 Chk2 Bloom s syndrome Werner syndrome RothmundThompson syndrome chromosomal instability Blm Wrn RecQ 8 9 Spindle assembly and polyploidization checkpoints NHF neo NHF E6 control colcemid P53 dependent G1 checkpoint function 10 ATM Nbs1 and Mre11 are required for the IRinduced S checkpoint NHF1 ATM 0 35 0 80 0 30 0 70 0 25 0 60 0 50 0 20 0 40 0 30 Normalized 3H CPM 0 15 0 10 0 20 0 05 0 10 0 00 0 00 0 10 20 Sham 30 0 10 1 5 Gy 20 Sham NBS1 30 1 5 Gy Mre11 0 70 0 60 0 60 0 50 0 50 0 40 0 40 0 30 0 30 0 20 0 20



View Full Document

Access the best Study Guides, Lecture Notes and Practice Exams

Loading Unlocking...
Login

Join to view DNA Damage and Repair and access 3M+ class-specific study document.

or
We will never post anything without your permission.
Don't have an account?
Sign Up

Join to view DNA Damage and Repair and access 3M+ class-specific study document.

or

By creating an account you agree to our Privacy Policy and Terms Of Use

Already a member?