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Biochemistry I Fall Term, 2004Lecture 12 (Handout): Ligand Binding MeasurementsC. Examples of Data AnalysisExperimental data, e.g. from an equilibrium dialysis experiment, can be analyzed to yield valuesfor Kd and n in several ways. Computer programs are available that provide automated andobjective estimates of these parameters. The processed data are then published in one or more ofthe following graphical formats:1. Saturation Curve: ν vs. [L].2. Scatchard Plot: single site (n = 1).3. Double Reciprocal Plot: single site (n =
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