(3rd ed.) Page 1 BIO 100 LAB SIGN OFF PAGE - UNIT 3 Name ___________________________ Please staple all of your lab pages for this Unit together with this page as the top. You will use this page to get your Labs for Unit 3 signed off by the Biology Learning Center staff. You need to have all of the following steps initialed by a staff member before you will be allowed to take the Unit 3 Exam. After you have obtained all of your sign offs for this Unit, be sure that a BLC staff member indicates on your Lab Card that you are OK to take the Unit 3 Exam. Also, keep this sign off page, along with your completed lab worksheets, as proof of your lab completion. If your Lab Card indicates that you have not completed the required Labs for this Unit, and you feel that you have, it is up to you to provide proof that you have indeed done the Labs. Keep this page! ___________ Unit 3, Step 2A (Enzymes - enzymes and lactose intolerance) ___________ Unit 3, Step 2B (Enzymes - enzymes and digestion) ___________ Unit 3, Step 2C (Enzymes - enzyme specificity) ___________ Unit 3, Step 2D (Osmosis - osmosis pre-lab sign off) ___________ Unit 3, Step 2E (Osmosis - osmosis experiment results) (*BLC STAFF: DO NOT SIGN OFF STUDENT UNTIL YOU HAVE RECEIVED THE STUDENT’S ENTIRE MOCK CELLS KIT!) *BLC Staff: After the student receives his/her last initial on this page, please record that he/she has completed the required lab signoffs for this unit.Page 2 of 8 NOTE: DO NOT ATTEMPT ANY OF THESE LABS UNTIL AFTER YOU HAVE READ THE TEXTBOOK CHAPTER AND VIEWED THE CORRESPONDING STUDY GUIDE! THEY PROVIDE THE NECESSARY BACKGROUND INFORMATION. Unit 3, STEP 2A: Enzymes and Lactose Intolerance lab This lab must be done in the Biology Learning Center. The instructions are located in Unit 3, Step 2A, of the BIO 100 Course Website. After you complete this lab, take your results to the front desk in the Biology Learning Center for a sign off (you will be prompted when to do so). To begin, go to Biology 100 Course Website, click on the Unit 3 link on the Course Content page, scroll down to find Step 2, and then click on the link to the “Enzymes and Lactose Intolerance” lab instructions. Follow the instructions on the website and fill in all of the required information on your worksheets as you complete the lab. TAKE YOUR COMPLETED WORKSHEETS TO THE FRONT DESK FOR A SIGN OFF BY A STAFF MEMBER BEFORE YOU MOVE ON TO PART B. DO NOT PUT ANY LAB MATERIALS AWAY UNTIL AFTER YOU HAVE RECEIVED YOUR CHECK OFF. (Unit 3, Step 2A)(3rd ed.) Page 3 Unit 3, STEP 2B: Enzymes and Digestion lab This lab must be done in the Biology Learning Center. The instructions are located in Unit 3, Step 2B, of the BIO 100 Course Website. After you complete this lab, take your results to the front desk in the Biology Learning Center for a sign off (you will be prompted when to do so). To begin, go to Biology 100 Course Website, click on the Unit 3 link on the Course Content page, scroll down to find Step 2, and then click on the link to the “Enzymes and Digestion” lab instructions. Follow the instructions on the website and fill in all of the required information on your worksheets as you complete the lab. jar number contents in tubing starch present outside tubing? glucose present outside tubing? 1 starch 2 glucose 3 saliva 4 starch + saliva 1. Does starch pass through dialysis tubing? 2. Does glucose pass through dialysis tubing? 3. What does the amylase in saliva do to the starch? 4. If the dialysis tubing represents the lining of the intestine, what has to happen to starch so that it can be absorbed by the body? TAKE YOUR COMPLETED WORKSHEETS TO THE FRONT DESK FOR A SIGN OFF BY A STAFF MEMBER BEFORE YOU MOVE ON TO PART C. DO NOT PUT ANY LAB MATERIALS AWAY UNTIL AFTER YOU HAVE RECEIVED YOUR CHECK OFF. (Unit 3, Step 2B)Page 4 of 8 Unit 3, STEP 2C: Enzyme Specificity lab This lab must be done in the Biology Learning Center. The instructions are located in Unit 3, Step 2C, of the BIO 100 Course Website. After you complete this lab, take your results to the front desk in the Biology Learning Center for a sign off (you will be prompted when to do so). To begin, go to Biology 100 Course Website, click on the Unit 3 link on the Course Content page, scroll down to find Step 2, and then click on the link to the “Enzyme Specificity” lab instructions. Follow the instructions on the website and fill in all of the required information on your worksheets as you complete the lab. test tube #1 #2 #3 #4 hydrogen peroxide 20 drops 20 drops -- -- starch -- -- 20 drops 20 drops iodine -- -- 2 drops 2 drops catalase 5 drops -- 5 drops -- amylase -- 1 ml -- 1 ml observations 5. Which enzyme breaks down hydrogen peroxide? 6. Which enzyme breaks down starch? 7. Define “enzyme specificity.” TAKE YOUR COMPLETED WORKSHEETS TO THE FRONT DESK FOR A SIGN OFF BY A STAFF MEMBER BEFORE YOU MOVE ON TO PART D. DO NOT PUT ANY LAB MATERIALS AWAY UNTIL AFTER YOU HAVE RECEIVED YOUR CHECK OFF. (Unit 3, Step 2C)(3rd ed.) Page 5 Unit 3, STEP 2D: Osmosis Pre-Lab This lab must be done in the Biology Learning Center. The instructions are located in Unit 3, Step 2D-E, of the BIO 100 Course Website. After you complete this lab, take your results to the front desk in the Biology Learning Center for a sign off (you will be prompted when to do so). To begin, go to Biology 100 Course Website, click on the Unit 3 link on the Course Content page, scroll down to find Step 2, and then click on the link to the “Membrane Transport: Osmosis” instructions. Follow the instructions on the website and fill in all of the required information on your worksheets as you complete the lab. Following the example in the pre-lab online instructions, identify the hypotonic solution (write “hypo”), hypertonic solution (write “hyper”), and draw an arrow indicating the direction that the water would move for each of the beakers (the rectangles) containing cells (the circles) below. Form your hypotheses based on the above pictures . In each space below, write either fresh, medium, or salty. 1. I hypothesize that when the cell with the medium salt concentration is placed in the ___________________ beaker, the cell will remain about the same weight.