Biological Chemistry Laboratory CHE 554, Spring 2012 Instructor: Dr. Anne-Frances Miller Office: Room 113 Phone: 7-9349 Email: [email protected] Web Site: http://www.chem.uky.edu/courses/che554/ Lab: Tuesday and Thursdays from 2:00 PM to 3:50 PM in CP-236 Lectures: From 1:00 to 1:50 in CP-222 (Tuesday and/or Thursday) Time slot will be used for laboratory exercises when not devoted to a lecture. Textbook: Experimental Biochemistry (3rd Edition), Switzer and Garrity. ISBN 0-7167-3300-5 Additional: Lab coat, safety glasses, USB memory stick, Lab notebook with duplicate pages Teaching Assistant Mr. Chris Noe [email protected] Mr. John Hoben [email protected] Details and Grading There are a total of 9 lab reports due in this class. The value of each report is listed at the end of this syllabus. All lab reports combined account for 70% of your final grade. Each of the two exams will count towards 10% of your final grade. The remaining 10% will reflect all measures of laboratory conduct. This includes safety issues, being prepared, being cooperative, being independent, professional orderly conduct etc. Lab reports are graded by Prof. Miller and detailed commentary is provided by a Teaching Assistant. Be sure that you understand the comments as these are provided to aid in improving the lab performance and reports. Information concerning how to write your reports will be provided separately. Reports not turned in on time, unless for approved excused reasons, will be given a 20% grade reduction for being 0.5-48 hours late, 40% for 2-4 days late, and 60% for less than 7 days but more than 4 days late. Reports more than 7 days (168 hours) late will not be graded. The Pre-lab section must be COMPLETE before the lab exercise begins. Students cannot begin working until their pre-lab section is complete, T.A.s have been instructed to annotate lab report to indicate pre-labs that were not complete at the beginning of the laboratory period. Students whose pre-labs are not complete at the beginning of the laboratory period may not be allowed to finish their work. T.A.s are not obligated to stay late for students who had not completed their pre-labs on time and students may not work in the laboratory without supervision of a T.A. or Prof. Miller. Attendance Because this is a laboratory class, and because all experiments are necessary to satisfy the requirements for ACS accreditation for this course, all students will be expected to complete all assignments. Attendance at official times is mandatory. We cannot provide TA supervision on an individual basis. After the first unexcused absence, each additional unexcused absence will result in a single letter reduction in your final grade. This includes lecture days. Anyone more than 10 minutes late to class will be considered absent and willnot be able to participate on that particular day. Because of the nature of biological chemistry experiments, they are difficult to make up on an individual basis. Office Hours Prof. Miller's office hours are 30 minutes after each lab and from 3-4 on Mondays. If you need to speak with Dr. Miller at any other times, you can stop by CP113 and see if she isavailable or you can make an appointment by email. "Remember, I am here to help you with this class. If you need help, I expect you to tell me." Course Material This class will use the textbook “Experimental Biochemistry” (3rd Edition), Switzer and Garrity, W. H. Freeman and Company, New York. You must have the textbook. Prof. Miller will also make available to you, in electronic form, all of the lectures. "I recommend that you print them out and have them with you on the lecture days. I will post them on the course web site at least 24 hours in advance of each lecture, or I will bring printed copies to class with me for you." Miscellaneous The student is responsible for all equipment they use. In other words, if you break it, you are responsible for replacing it. The student will also be responsible for obtaining a lab coat (non-synthetic), a lab notebook, and a USB memory stick. Make sure the notebook has the yellow pages (duplicates). Lab coats and goggles have to be worn in the lab at all times. You must wear proper footwear and clothes that provide good coverage. Cell phones need to be turned off except for pre-approved reasons. Anyone behaving in an unsafe or disrespectful manner in the lab will be made to leave the lab and a grade of zero assigned on the given lab assignment. Cheating and plagiarism in any form will not be tolerated, and will result in a grade of zero for the final grade of the course. Please note that cheating takes many forms. You are not allowed to share data. You are allowed to discuss your experiments with others, and to get tips from others or watch how they do something, but the reports must be 100% your own data, your own words, and your own answers and conclusions. Everyone must execute their experiments and reports themselves completely independently of others. Cameras will be available for students to use to document experimental apparatus set-up and results. However students preferring to use their own camera are welcome to do so. Photo-documentation of procedures, data and analysis need to be taped into the lab report and should be present in both the official report that will be turned in as well as the copy that will be retained.Lab Reports Lab reports are due before class starts on the day that is 2 weeks after the date on which the experimental activities were completed in lab, except for the last two lab reports which are due on 27 April by midnight. Pages are to be in order and stapled. Reports are to be put in Prof. Miller's faculty mailbox, on top of other mail. The lab report is the set of original laboratory notebook pages, pulled from the notebook. The intention is that nothing need be copied over. Duplicate pages are the student's personal copy of the work, which will hopefully prove to be a useful resource in the future. All computer outputs such as graphs, charts and printed photos should therefore be printed in duplicate so that one copy can be taped into a designated spot in the original sheet (to be turned in) and another can be taped into the duplicate sheet (to be retained). Report Points Laboratory Assignment #1 Spectrophotometry 10 points Laboratory Assignment #2 Chromatography 10 points Laboratory Assignment #3