BIOCHEMISTRY LAB (554)SyllabusProfessor TestaBiological Chemistry LaboratoryTentative Schedule (Spring 2008)Instructor: Dr. Stephen TestaOffice:Room 319 Phone: 7-7076Email: [email protected]: Tuesday and Thursdays from 1:00 PM to 3:50 PM in CP-236Lectures: TBA from 1:00 to 2:00 in CP-211Textbook: Experimental Biochemistry (3rdEdition), Switzer and Garrity.Teaching AssistantSarita HardasDetails and GradingThere are a total of 7 lab reports due in this class. The worth of each report is listedat the bottom of this syllabus. All lab reports combined account for 75% of your finalgrade. Each of the two exams will count towards 10% of your final grade. The remaining5% will be up to the discretion of the instructor and/or the lab TAs regarding all measuresof laboratory conduct. This includes safety issues, being prepared, being independent,etc.Lab reports are graded by the TAs, although any comments or concerns you haveregarding such grading should be brought to your instructor‟s attention (after firstdiscussing the matter with your TA). Information concerning how to write your reports willbe provided separately. Reports not turned in on time, unless approved for excusedreasons, will be given a 33% grade reduction for being 0.25-24 hours late, 66% for 24-48hours late, and 100% thereafter.Because this is a 500-level class, differentiation must be made between theexpectations for undergraduate and graduate students. Because this is an introductorylaboratory class, and because all experiments are necessary to satisfy the requirementsfor ACS accreditation for this course, all students will be expected to complete allassignments. Therefore, this differentiation will manifest itself in the following ways:1) Undergraduate reports will be graded with more of a focus on understanding theexperiments (the results will be deemphasized).2) Undergraduates will not have to answer all the test questions, but a defined subset.AttendanceAttendance is mandatory. After the first unexcused absence, each additionalunexcused absence will result in a single letter reduction in your final grade. This includeslecture days. Anyone more than 10 minutes late to class will be considered absent andwill not be able to participate on that particular day. Because of the nature of preparing forbiological chemistry experiments, they are difficult to make up on an individual basis.Therefore, students will be permitted to make up excused absences by completing thealternative experiments planned on the days marked „TBA‟. Note that these experimentscould include the experiment that was missed. Note: I am very strict with attendance, soplease attend all classes.Office HoursMy office hours are 30 minutes before and after each class. If you need to speakwith me at any other times, you can stop by my office and see if I am available or you canmake an appointment. Remember, I am here to help you with this class. If you need help,I expect you to tell me.Course MaterialThis class will use the textbook “Experimental Biochemistry” (3rdEdition), Switzerand Garrity, W. H. Freeman and Company, New York. You must have the textbook. I willalso make available to you, in electronic form, all of my lectures. I recommend that youprint them out and have them with you on the lecture days.MiscellaneousThe student is responsible for all equipment they use. In other words, if you breakit, you are responsible for replacing it. The student will also be responsible for obtaining alab coat, a lab notebook, a memory stick, and a combination lock (you must give me thecombination). Make sure the notebook has the yellow pages you can rip out and hand in.Lab coats and goggles have to be worn in the lab at all times. Cell phones need tobe turned off except for pre-approved reasons. Anyone behaving in an unsafe ordisrespectful manner in the lab will be made to leave the lab and a grade of zeroassigned on the given lab assignment.Cheating in any form will not be tolerated, and will result in a grade of zero for thefinal grade of the course. Please note that cheating takes many forms. I will summarizewhat I expect: everyone must work on their experiments and reports completelyindependent of others. You are not allowed to share data. You are allowed to discussyour experiments with others, but the reports must be 100% your data, your words, andyour answers and conclusions.Biological Chemistry LaboratorySpring 2008 ScheduleJanuary 10 (Week 1; Day 1): Introduction to this courseJanuary 15 (Week 2; Day 1): Safety and setting upJanuary 17 (Week 2; Day 2): Learn basic techniquesJanuary 22 (Week 3; Day 1): Lecture on Photometry (lab prep)January 24 (Week 3; Day 2): Laboratory Assignment # 1: Photometry - Protein Absorption (Exp 1)January 29 (Week 4; Day 1): (*) Lecture on Chromatography (lab prep)January 31 (Week 4; Day 2): Laboratory Assignment # 2: Chromatography - Protein Separation (Exp 2)February 5 (Week 5; Day 1): (*) Lecture on ELISA (lab prep)February 7 (Week 5; Day 2): Laboratory Assignment # 3: ELISA (Exp 17)February 12 (Week 6; Day 1): (*) Lecture on Kinetics (lab prep)February 14 (Week 6; Day 2): Laboratory Assignment # 4: Properties of -Galactosidase (Exp 7)February 19 (Week 7; Day 1): Laboratory Assignment # 4: Properties of -Galactosidase (Exp 7)February 21 (Week 7; Day 2): ReviewFebruary 26 (Week 8; Day 1): Exam #1February 28 (Week 8; Day 2): (*) Lecture on the Polymerase Chain Reaction (lab prep)March 4 (Week 9; Day 1): Laboratory Assignment # 5: The Polymerase Chain Reaction (Exp 24)March 6 (Week 9; Day 2): Laboratory Assignment # 5: The Polymerase Chain Reaction (Exp 24)March 11 Spring BreakMarch 13 Spring Break March 18 (Week 10; Day 1): (*) Lecture on Affinity Purification, SDS Page, Western Blot (lab prep)March 20 (Week 10; Day 2): Laboratory Assignment # 6: Purification of GST(Exp 10)March 25 (Week 11; Day 1): Laboratory Assignment # 6: Western Blot to Identify an Antigen (Exp 18)March 27 (Week 11; Day 2): Laboratory Assignment # 6: Western Blot to Identify an Antigen (Exp 18)April 1 (Week 12; Day 1): (*) Lecture on Molecular Biology (lab prep)April 3 (Week 12; Day 2): Laboratory Assignment # 7: Molecular Biology (Cloning)April 8 (Week 13; Day 1): Laboratory Assignment # 7: Molecular Biology (Cloning)April 10 (Week