U-M SI 501 - Recombinant DNA Technology (27 pages)

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Recombinant DNA Technology



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Recombinant DNA Technology

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Pages:
27
School:
University of Michigan
Course:
Si 501 - Contextual Inq

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Recombinant DNA Technology Stephen B Gruber MD PhD Division of Molecular Medicine and Genetics November 4 2002 Learning Objectives Know the basics of gene structure function and regulation Be familiar with the basic methods of molecular genetics Understand the meaning of DNA sequence and amino acid polymorphisms Know how DNA sequence analysis is performed and be familiar with methods of screening for differences Have a general understanding of methods for gene transfer into tissue culture cells and the power of transgenic technologies 1 Learning Objectives 1 Know the basics of gene structure function and regulation Be familiar with the basic methods of molecular genetics Understand the meaning of DNA sequence and amino acid polymorphisms Know how DNA sequence analysis is performed and be familiar with methods of screening for differences Have a general understanding of methods for gene transfer into tissue culture cells and the power of transgenic technologies Chromosomes DNA and Genes Gene Cell Nucleus Chromosomes Protein Adapted from Understanding Gene Testing NIH 1995 2 Genetic Code A codon is made of 3 base pairs 64 codons total 1 codon AUG encodes methionine and starts translation of all proteins 61 codons encode 20 amino acids redundant code 3 codons stop protein translation A U G G C A U A A Met Ala DNA Transcription and Translation Growing chain of amino acids mRNA Ribosome DNA Nuclear membrane Protein Cell membrane Adapted from Understanding Gene Testing NIH 1995 3 Gene Structure RNA transcription start site Splice sites Stop site Promoter Exon 1 Intron Exon 2 Intron Exon 3 5 end 3 end Exon 1 Exon 2 Exon 3 mRNA RNA Processing Exon Intron Exon Intron Exon DNA Transcription Primary mRNA GU AG Processing Mature mRNA Translation Protein 4 Learning Objectives 2 Know the basics of gene structure function and regulation Be familiar with the basic methods of molecular genetics nucleic acid hybridization Southern DNA and northern RNA blotting PCR DNA sequencing



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