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ADSC 2010: Test 2

Nutrition
The study of feedstuffs and their efficient utilization by livestock in producing animal products such as: Meat Wool Milk Work 
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What is the largest production cost?
Feed Costs
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Nutrient
A food constituent (a chemical substance) that is absorbed from the digestive tract and is metabolized by he body to support life 
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6 Classes of Nutrients
Water Fats  Carbohydrates  Vitamins  Minerals  Proteins
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Where does an animal get essential proteins that their body cannot produce?
Diet 
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How much of the body weight consists of water?
70=75%
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Functions of Water
Transportation Media for Metabolic rxns Thermoregulation  Lubricant  Gives cells their shape 
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What is the most highly consumed nutrient?
Water 
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Average daily consumption of water for swine
1.5-3 gal/day
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Average Daily Consumption of Water for Cattle
10-14 gal/day
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Average Daily Consumption of Water for Sheep
1-3 gal/day 
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Average Daily Consumption of Water Per Day for Horses
10-14 gal/day
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What lack of nutrient will animals die quickest from?
Water
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Primary components and function of Carbohydrates
Carbon, Hydrogen, and Oxygen  To provide energy 
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How do we measure energy?
Calorie (cal) Kilocalorie (Kcal) = 1000 calories Megacalorie (Mcal) = 1mil calories 
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Energy content of one carb
4.2 Kcal/g 
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Classification of Carbohydrates (CHO's)
Monosaccharides  Disaccharides  Polysacharides 
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Monosaccharides
Simple Sugars (5-6 Carbons)  Glucose (Glu) (dextrose)  Fructose (Fru) Galactose (Gal)  Ribose/Deoxyribose 
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Disaccharides
Sucrose Glu + Fru alpha 1-2 linkage  Lactose  Glu + Gal  beta 1-4 linkages  Maltose  Glu + Glu  Alpha 1-4 linkages 
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Polysaccharides
Starch Glycogen  Cellulose  Hemicellulose  Lihnin 
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Starch (GLU)n
Bonded by alpha 1-4 linkages  Energy Storage in plants  Primarily in Concentrates 
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Concentrate
High energy, low fiber feedstuff that is highly digestible  Ex: Corn, Grain, Sorghum, Wheat 
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Glycogen
Animal Starch  Stored in liver and muscle 
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Cellulose
Bonded by beta 1-4 linkages  Found in the cell wall of plants  Lowly digestible  Primarily in roughage
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Roughage
High fiber, low energy feedstuff  lowly digestible 
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Hemicellulose
alpha and beta 1-4 linkages 
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Lihnin
Indigestible 
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What role do linkages play?
They determine whether something is digestible or indigestible 
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What can animals break down?
Cellulose and Starch 
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Functions of Carbohydrates
Energy Source  4.2 Kcal/g  50-75% of the dry matter may be carbohydrates  Fiber Content  Laxative Effect  Maintains healthy lining and muscle in the digestive tract 
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Fats and Lipids main components
Carbon, Hydrogen, and lower proportion of Oxygen than Carbs 
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Classification of Fats and Oils
Simple Lipids: Triglycerides  Compound Lipids  Sterols 
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Simple Lipids
Triglycerides  Glycerol + 3 fatty acids  Saturated Fatty Acid  No Double Bonds  Unsaturated fatty Acids  At least one double bond
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How to Name Fats and Lipids
Carbon:Double Bond Ratio  Ex: 18 Carbon Atoms and 2 Double Bonds  C18:2 
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Physical form of fats and lipids
Solid (Fats): Primarily saturated and(or) long-chain fatty acids  Liquid (Oils): short chain and(or) unsaturated fatty acids  Dietary lipids can impact the physical characteristics of carcass fats; especially in "monogatrics"
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Dietary Requirements
Very low requirement for fats; however there are: Essential Fatty Acids  Linoleic acid (18:2)  Linolenic acid (18:3)  Archidonic acid (20:4) 
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Compound Lipids
Glycolipids: Nervous tissue  Lipoproteins: Involved in lipid transportation  Phospholipids Vitamin Precursors 
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Sterols
Cholesterol: Involved in membrane structure and a precursor for other compounds  Steroid Hormones  Vitamin Precursors Vitamin D (ergosterol + sunlight) 
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Functions of Fats and Lipids
Storage form of metabolic fuel  9.45 Kcal/g  Transportation of Energy  Aid the absorption of vitamins  Protection and Insulation of vital organs  Hormone Precursors  Juiciness and flavor in meats 
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What % of feedstuff is fat lipid?
Generally contain 1-4% fat lipid except for oil seed which may contain up to 18% (soybeans, canola, cottonseed) 
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Protein main component and main function
Carbon Hydrogen Oxygen Nitrogen  Sulfur  To provide building blocks for animal proteins 
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Essential Amino Acids
Methionine  Arginine  Threonine  Tryptophan Histidine  Isoleucine Leucine Lysine Phenylalanine
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Semi-Essential Amino Acids
Glycine Tyrosine  Cysteine 
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Non-Essential Amino Acids
Alanine  Glutamine  Serine  Proline  Aspartic Acid Glutamic Acid Asparagine 
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Proteins are chains of amino acids linked by ________?
Peptide Bonds 
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Functions of Proteins
Structure: Muscle, Hair, Feathers, Cell wall  70-80% of dry, fat free body is protein  Regulation and protection  Enzymes  Hormones (rSBT): Fairly small proteins that bring about a specific response in the body, Growth hormones put into cows produce very little milk, no such thing as hormone free milk and meat  Antibodies           Hemoglobin
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Protein Energy
4 cal/g 
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Protein Quality
Determined by the ability of an animal to digest and utilize the protein  Proportions of the essential amino acids are similar to animal needs 
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Dietary Crude Proteins
Crude Protein of feedstuff is calculated based on nitrogen content 
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Calculating Crude Protein
% crude protein = 6.25 x % nitrogen  ex: How much CP is in 4% N? 4% x 6.25 = 25%
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Non-Protein Nitrogen
Examples: Urea and Uric Acid  Mammals cannot use this form of Nitrogen; however the microorganisms in the rumen can  Excess Urea is toxic 
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Vitamins
Micronutrients  Only needed in small amounts  Large Organic Molecules 
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Vitamin Categories
Water Soluble  Fat Soluble  Salt Soluble 
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Fat Soluble
A, D, E and K 
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Water Soluble
Thiamin (B1)  Bictin Choline  Cyanocobalamin (B12)  Riboflavin (B2) Pyridoxine (B6)  Folic Acid  Pantothenic Acid 
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Vitamin Requirements
Monogastrics need allll of the vitamins  Ruminants  Need fat soluble only except k  They can make B vitamins and K by themselves  The bacteria in the rumen produces water soluble  Non-protein nitrogen can help produce needed amino acids 
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Vitamin Functions
Metabolism  Cofactors and Coenzymes  Help regulate  Absorption and Digestion  D: Ca/ P absorption (added to milk to help absorb Ca)  B: Aids in protein digestion  A: Important in maintaining body linings  K: Blood clotting factor  D: Prevent white muscle disease and aid bone metabolism 
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White Muscle Disease
Issue with Calcium and Phosphorous in the muscle 
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Minerals
Micronutrients  Chemical elements other than C,H,O, and N needed for metabolism  Needed in small amounts 
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Mineral Categories
Macro  Required in larger quantities than Micro's  Ca, P, Na, K, Cl, Mg, S Micro (Trace) Cr, Co, Cu, I, Fe, Mn, Zn (F, Mb, Se)  Don't Confuse 
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Mineral Functions
Structure Bone Growth (Ca & P)  Metabolism  Cofactors and Coenzymes (Zn)  Energy Storage  Fluid Balance  Part of other compounds  Nerve and Muscle Functions 
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Categories of Feedstuff
Concentrates Roughage
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Concentrates
High in energy, low in fiber Fairly Digestible  Cereal Grains  Oil seeds (crops and meal)  By-Products 
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Cereal Grains
Corn  Oats  Barley Rye Tritcale Sorghum  Wheat  Excellent source of energy from carbs and starch  Moderate to low in protein (have to add concentrates)  Poor Ca and vitamin A source  Moderate source of P 
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Oil Seeds
Soybean  Cottonseed  Linseed Outstanding Protein source  Good Source of energy and P  Fair to low in Ca Poor source of Vitamin A
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Soybean
Fed soybean mean rather than whole soybean  Extract oil out and then what is left is used in meal  Largest source of protein in animal feed  Legume Plant 
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Cottonseed
Fed the whole seed  Routinely produced in Georgia  High in oil/energy  Also ground but not as effective 
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By-Products of Concentrates
Dried beet/citrus pulp Molasses Dried Bakers Waste Whey Products  Animal fat  Chicken Litter 
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Roughage
Low in energy, High in fiber  Legumes  Grasses 
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Roughage Legume
Nitrogen Fixing  Clovers  Alfalfa  High in Protein  Moderate to low in energy  Good Calcium and vitamin A source  Moderate source of P 
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Grasses
Bermudagrass (North)  Bahiagrass (South) Fescue (North to South)  Most pastures here  Causes issues in horses with gestation and pregnancy (miscarriages)  Low in energy  Moderate to low in protein  Good source of vitamin A and calcium  Moderate to low in P 
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Forms of Roughage
Pasture: feed harvested by animal  Hay: roughage is cut, air-dried, baled and stored  Silage: hayleage and baleage - roughage is cut and stored wet to cause fermentation  fermentation causes pH reduction that results in preservation of roughage  put in anaerobic environment to prevent mold  Corn and sorghum used: silage (whole plant) used in dairy industry 
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Ferment
Breaks down sugars and produces acid 
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Proximate Analysis of feedstuff
The separation of feed components into categories based on their feeding value 
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6 Components of feedstuff
Moisture  Crude Protein Crude Fat  Crude Fiber Nitrogen-free extract  Ash 
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As-fed
How we feed the animal 
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Breakdown of feed
Feed: 100% Moisture: 12%  Dry Matter: 88% Ash: 5% Organic Matter: 83%  Crude Protein: 13%  Non-nitrogen Minerals: 70%  Crude Fat: 4%  CHO: 66%  Crude Fiber: 10%            Nitrogen Free Extract: 56% 
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Crude Fiber
Lignin  Hemicellulose 
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Digestibility of Feedstuff
The amount of nutrient that is absorbed in the digestive tract 
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Nutrient In-Feed Calculation
Nutrient in feces/nutrient in food x 100%
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Which nutrients contribute to the energy content of feeds?
CHO's, lipids, and proteins 
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Methods to estimate the energy content of feeds
1. Total digestible nutrients (TDN) 2. Metabolizable Energy (ME) and Net Energy (NE) 
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TDN %
Based on the proximate analysis and digestibility measures for a feed  Calculation: % digestible protein + % digestible fat x 2.25 + digestible crude fiber  + % digestible NFE
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Factors affecting TDN
Dry Matter Content  Nutrition Digestibility  Ash Content  Fat Content 
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...
Gross energy (Kcal/g or Mcal)  Digestible Energy  Metabolizible Energy  Cattle and sheep lose most energy through gas because they are ruminant  Collect urine and gas to measure  Net Energy Maintenance  Production 
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Maintenance Requirements
Maintain the tissue balance of the animal (no gain, no loss)  Provide adequate energy for normal activity  Replace nutrients lost through basal metabolism  Maintenance requirements are based on size and metabolic weight of an animal  About half of the nutrient fed to livestock is used for maintenance requirements  Different for every animal to keep zero balance  Younger animals need nutrients fro growth rather than maintenance 
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Nutrient Utilization for Production
Nutrients provided in excess for maintenance are used for production  Growth: need building block to make new tissues for energy and for constructing skeletal and muscle growth  Reproduction: needed for gamete production and fetal growth Milk and Wool production Work 
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Digestive Systems: Monogastrics
Mouth - Esophagus - Stomach - SI - Bile Duct - Key Junction: Cecum & LI - rectum 
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Esophagus: Monogastric
Passageway for food to stomach  Horses have issues with their esophagus due to long necks 
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Stomach: Monogastric
Storage and digestion of food  Muscle contractions cause physical breakdown  HCl lowers pH of stomach, kills most bacteria, and activates  enzymes: pepsin and renin HCl takes a portion of the protein off  In the phyloric sphincter (posterior end) 
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Enzymes: Monogastric
Pepsin: Protein degradation  Causes a break in protein Renin: coagulates milk protein; limited amount of lipase activity 
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Small Intestine: Monogastric
Stomach mixes feed well and initiates limited digestion, feed mixture moves into SI (Chyme)  Main Part of digestion and absorption  3 sections  1. Duodenum - 1st part 2. Jejunum - 2nd part 3. Ileum - 3rd part 
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Mouth: Monogastric
Chewing: want small particles; easier for digestion  Salvation and Mucin act as a lubricant  Amalyse breaks down starches  Horses don't produce HCO4 buffers the end of the stomach where the esophagus ends: very acidic 
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Chyme
Food as it leaves the stomach 
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Digestion in the SI
Polypeptides - amino acids  Starch - Monosaccharides  Basic Unit of Starch: Glucose  Lipids - Fatty Acids 
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Digestive Organs associated with SI
Gall Bladder: secretes bile produced in the liver Bile: encapsulates and degrades fats Pancreas: secretes enzymes into the duodenum to break down macro molecules  also secretes insulin and glycogen to control blood-glucose levels
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Human Prehension
Hands
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Horse Prehension
Lips
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Cow Prehension
Tongue 
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Sheep/Goat Prehension
Tongue and Lips
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Chicken
Beak 
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Prehension
How an animal collects food
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Mastication
Chewing
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Horse Mastication
Teeth on upper and lower mandible  Can only chew on one side at a time 
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Cow Mastication
Dental pad on bottom 
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Ruminant Characteristics
Chew Bolus 4 Compartments in stomach: much more room than monogastric Reticular Groove
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Monogastric Issues
Small stomach compared to body  Can overfeed and cause issues  Colic Herbivore
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Bolus
Soft mass of chewed food; cud
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What is a young ruminant considered?
A monogastric 
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Proximal Colon
LI much bigger than stomach: Cecum
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Colic
A digestive disorder (small stomach, big appetite) 
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Pig Digestive Tract
Mouth > Esophagus > Stomach > Small Intestine > Bile Duct > Key Junction, Cecum; LI > Rectum 
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What secretes bile? What stores it?
Liver secretes  Gall Bladder stores 
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What does the pancreas secrete?
Digestive Enzymes 
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Poultry Digestive System
Esophagus > Crop > True Stomach > Proventriculus > Gizzard > SI > Pancreas > Gall Bladder > Ceca > LI > Urinary Tract > Cloeca 
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Crop
Storage of large particles 
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True Stomach/Proventriculus
Acid is secreted and digestion begins 
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Gizzard
Breakdown of particles and grains 
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Cecum
Fermentation odor, particle size decreases, thin wall 
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Cattle Digestive System
Esophagus > Rumen > Reticulum > Omasum > Abomasum > SI > T-junction with Cecum and LI > Rectum 
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How do cows contract hairballs?
From eating things they shouldn't, licking themselves, and licking others 
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Genetics
Science concerned with the study of heredity and variation. Allows us to identify animals that will excel in certain traits and ones that do not  Shows us how to change a gene in a herd
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Heredity and Variation
Heredity is why we look like our parents  Variation is why we don't 
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How is genetic material passed from parents to offspring?
It is passed through reproductive cells; sperm and ova  Creates a link from generation to generation 
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Variation
The occurrence of differences among individuals of the same species
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Within the Nucleus
Chromosomes Genes DNA Nucleotides Bases
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Chromosomes
Nuclear structures that carry the genes containing genetic material 
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Genes
The basic unit of inheritance  Found in a fixed location on the chromosome  A defined unit of DNA composed of a certain nucleotide sequence 
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DNA
Deoxyribonucleic Acid A double-stranded molecule composed of nucleotides 
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Nucleotides
Composed of:  A pentose sugar or 5-sided ring Phosphate  A nitrogenous base 
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Bases
Classified as:  Purines : Thymine and Cyosine  Pyrimidines: Adenine and Guanine 
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Transcription of DNA
Also called replication mRNA is formed from DNA  Occurs in the nucleus  mRNA is single-stranded  Sequence is complimentary  Uracil replaces thymine  mRNA is very unstable  mRNA is transported out of the nucleus for translation 
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Translation of mRNA to Protein
Ribosome is formed (rRNA)  mRNA attaches to the ribosome, providing the base sequence for a specific protein: Codon; 3 base sequence  tRNA attaches to specific amino acid based on a 3 base sequence: anticodon  tRNA + AA (anticodon) interacts with mRNA (codon) and amino acids are linked by peptide bonds to the growing protein  Used tRNA is released 
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Chromosome
Occur in pairs in mammalian cells and the number is constant for a species. All cells have a full set (paired) of chromosomes (diploid, 2n) except reproductive cells (haploid, n); one copy For each pair , one originated in the paternal and one originated in the maternal  Half the chromosomes from each parent 
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Man Chromosome Numbers
2n: 46 23 pairs
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Cattle Chromosome Numbers
2n: 90  60 pairs 
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Swine Chromosome numbers
2n: 38 19 pairs 
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Horse
2n: 64  32 pairs 
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Sheep Chromosome Numbers
2n= 54  27 pairs 
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Sex Chromosome
Determines the sex of the animal (x and y) 
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Is the X or Y chromosome larger?
X chromosome is 2-3 times larger than the Y 
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XY
Male
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XX
Female 
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Who decides the sex of the offspring?
The male (50/50 chance) 
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Mitosis
Generating another cell The process of cell division where two identical daughter cells are formed with the same compliment of chromosome pairs (diploid) 
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Steps of Mitosis
1. Chromosomes duplicate and appear double-stranded (each strand is a chromatid)  2. Chromatids are attached to their duplicate by the centromere  3. Nuclear Membrane disappears  4. Spindle fibers form and attach to centromeres  5. Chromosomes line up at the center of the cell and centriole forms  6. The chromatid pairs separate and move towards centriole  7. Nuclear membrane reforms around the centrioles  8. Cell divides
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Meiosis
Forms reproductive cells  The process of cell division where daughter cells (sperm or ova) contain one-half the number of chromosomes (haploid) 
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First Division Steps of Meiosis
First Division:  1. Chromosomes duplicate into chromatids  2. Homologous pairs of chromosomes pair up - tetrads  3. Spindle fiber forms, centriole forms  4. Tetrad separates and homologous chromosomes migrate to poles  5. Cell divides (diploid) 
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Second Division Steps of Meiosis
6. Chromatids of each chromosome separate  7. Cell divides (haploid) 
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Gametogenesis
The production of gametes or reproductive cells Spermatogenesis and Oogenesis 
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Spermatogenesis
Male reproductive cell production Primordial germ cell (diploid) > Primary spermatocyte (tetraploid) > Secondary Spermatocyte (diploid) > Spermatid (haploid) > Mature Sperm (4 sperm cells) 
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Tetraploid
4 copies have gone through replication
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Oogenesis
Female reproductive cell Production Primordial Germ Cell (oogonia; diploid) > Primary Oocyte (splits in two, one goes to polar body and does not continue you through process; tetraploid) > Secondary Oocyte (diploid; splits in two groups also) > Ootid (haploid)  > Ovum (1 cell) 
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Gene Locus
Specific location of a gene on a chromosome Forms the coding system that directs the production of enzymes and proteins; and thus controls the development of all traits 
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Homologous Chromosomes
Have the same location on both copies 
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Allele
Different forms of a  gene that can occur at the same locus  Ex: Coat Color  B = black  b = red 
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Homozygous
The alleles at the homologus gene sites are identical 
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Heterozygous
The genes at the homologous gene sites are NOT identical 
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Genotype
Genetic make-up of an individual as determined by its genes 
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Phenotype
Physical expression of an individual's genotype  The result of gene content and environment: allele Phenotype = Genotype + environment 
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Father of Genetics
Gregor Mendel  Austrian Monk  1st to understand inheritance  He saw changes in phenotypes and that helped him to understand his two principles  Principle of Segregation  Principle of Independent assortment 
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Principle of Segregation
Paired genes (alleles) are separated from each their and distributed in different sex cells One copy, only one 
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Principle of Independent Assortment
Alleles are separated independently into sex cells. Each allele has an equal chance of being included in a gamete 
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Two types of inherited traits
Qualitative and Quantitative 
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Qualitative traits
Traits that are controlled by 1 or 2 genes   Coat Color  Horns Cryptorchidism - male with one testicle  Inverted Nipple 
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Quantitative Traits
Traits that are affected by many genes and contain a continuous variation Harder to predict  Tend to have the largest economic impact  Rate of gain  Feed efficiency  Milk Production  Carcass Quality  Fertility 
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Inheritance
Each allele will have a different effect on phenotype  Dominant and Recessive Alleles 
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Dominant Allele
This allele expresses itself or limits the expression of other alleles 
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Recessive Allele
The allele whose expression is limited 
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Homozygous Dominant Allele Example
BB
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Heterozygous Allele Example
Bb
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Homozygous Allele Example
bb
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Phenotypes affected by dominant and recessive alleles
Coat Color  Polled or Horned Mule Foot Double-muscling  Dwarfism  Homozygous recessive offspring usually don't survive
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Scurs
Horn-like tissue that is attached to the skin of polled cattle; not attached to skull 
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Codominance
Neither allele completely covers the other, thus the phenotype is a mixture or blending  Ex: Coat Color in Shorthorn RR: red  WW: White  RW: Roan 
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Epistasis
A situation in which a gene or gene pair or gene pair masks (or controls) the expression of another non-allelic gene pair  Ex: Albinoism in cats  Two pairs of alleles: C - color, c - albino               B - black, b - brown CC or Cc then B allele determines color : Black or brown  cc then C allele determines color 
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Overdominance
A situation in which the heterozygote is superior to either of the homozygotes 
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Heterosis
Or hybrid vigor  Performance of the offspring that is superior to the average of the parents  Due to overdominance  Ex: milk production, growth traits, reproduction 
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Two Basic Methods for Genetic Improvement
Selection of outstanding individuals  Mating or breeding systems 
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Selection
Choosing which animal(s) in a generation will be allowed to become parents in the next generation and thus be the source of genetic material for the population  Changes the allelic "frequency" of a gene in the population 
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Methods of Selection
1. Single Trait Selection  2. Tandem Selection  3. Independent Culling Level 4. Selection Index
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Single Trait Selection
Focus on improving one single trait  Results in undesirable traits along with it 
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Tandem Selection
Select for a trait until some desired level is reached then select for another trait  Multiple Trait Improvement 
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Independent Culling Level
Set minimum standards for a trait and cull animals that fail  If they don't meet the standard you set, get rid of them 
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Selection Index
Combines traits into one value, based on their economic importance and level of genetic variation Moving towards in today's industry  How much change can be made along with economic importance; must be reasonable 
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Selection Tools
1. Appearance  2. Pedigree 3. Family Selection  4. Individual Performance Records  5. Progeny Testing  6. Genotyping 
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Heritability
Portion of phenotypic variation that is due to genetic causes and can be passed to offspring 
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Selection Tools: Appearance
Eliminate physical abnormalities  Select for certain conformation (shape of animal)  Structural Soundness
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Selection Tools: Pedigree
Record of ancestry (family tree)  Identifies lines that excel in traits 
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Selection Tools: Family Selection
Selection based on the performance of the entire family/poor performing families are culled 
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Selection Tools: Individual Performance Records
Information on the individual  Trait has to be moderately to highly heritable  Test trait of interest  Downside: Have to wait until the trait is expressed in offspring before you make a genetic decision 
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Selection Tools: Progeny Testing
Evaluation of an individual based on its offspring  Must wait for gene to be expressed in offspring 
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Selection Tools: Genotyping
Looking at genes and identifying anomolies and absence or presence of markers that define a certain trait (will not change)
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What is the object of using selection tools?
To develop "breeding values" 
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Breeding Value
A measure of the genetic value of an animal 
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Transmitting Ability
Breeding value / 2  The amount an individual can contribute to its offspring 
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Two main forms of Transmitting Ability used in the livestock industry
Expected Progeny Difference (EPD)  Predicted Transmitting Ability (PTA)  Based on records from: Individual  Pedigree  Progeny  The more info we utilize, the better the prediction 
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Requirements to make genetic progress
1. Trait must be inheritable  The more heritable the quicker we can make change  2. Must be variation in the population  Genetic Progress = h^2 x SD(selection differential) 
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Selection Differential
Difference between the average of the "individuals selected for mating" and the "population" 
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Mating Systems
Organized manner of mating the "selected" animals  1. Random 2. Inbreeding  3. Outbreeding 
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Random Mating
No selection 
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Inbreeding
Mating in animals that are more closely related than the average of the population  Reduction in genetic variation 
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Line Breeding
Concentrate the genotype of one superior animal  Male parent to granddaughter or mother to grandson  Concentrate on traits 
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Inbreeding Depression
Reduction of genetic variability and thus reduced vigor and fertility 
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Outbreeding
Also called crossbreeding  Mating of animals that are less closely related than the average of the population
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How do we combine selection and mating systems?
Cross females to increase prolificacy, then mate with a meaty male to increase meat factor in offspring 
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Crossbreeding Systems
1. Two Breed Tewrminal Cross 2. Two Breed Rotational Cross 3. Three Breed Rotational Cross 4. Three Breed Rota-Terminal Cross 
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Two Breed Terminal Cross
Advantages:  Simple Maximized heterosis  Disadvantages:  Have to maintain purebred  No heterosis in females 
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Two Breed Rotational Cross
Advantages:  Simple Produce Females Heterosis in dam Disadvantages:  67% heterosis  Two pastures needed  Two herds produced Once a female moves herds it does not move again or return to original herd
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Three Breed Rotational Cross
Advantages:  86% heterosis  Produce females  Heterosis in dam  Disadvantages: Large #'s needed  Multiple Pastures  Labor and Management Intensive  The more breeds we have the larger the % of heterosis
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Two Breed Rota-Terminal Cross
Advantages:  Produce replacement females Heterosis in females Maximum heterosis in terminal cross calves  Can utilize complimentarity in terminal cross calves  Disadvantages:  Only 67% heterosis in females and rotational calves  Requires greater number of animals to implement  Requires greater management input 
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