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In Eukaryotic cells, glycolysis occurs in the __, and the TCA cycle occurs in the
cytoplasm; mitochondria
The pyruvate dehydrogenase complex contains three multimeric enzymes (E1, E2, and E3). All are properties of E1 subunit EXCEPT:
It binds NAD+ in its active site
Properties of E2 include:
It uses thiamin pyrophosphate as a catalytic coenzyme it oxidatively decarboxylates pyruvate it transfers an acetyl group to lipoamide of Eta it forms a hydroxyethyl-TPP intermediate
10. Which is NOT true of citrate synthase? A. it catalyzes the first reaction in the TCA cycle B. it is not regulated C. OAA and acetyl-CoA bind to the active sites D. the citrate synthase reaction liberates a relatively large amount of energy E. all of the above are true
B It is not regulated
In the TCA cycle __ oxidation uses NAD+ as the oxidizing agent while __ oxidation uses FAD as the oxidizing agent
3; 1
a-Ketoglutarate dehydrogenase is a multi-enzyme complex analogous to:
pyruvate dehydrogenase
The correct sequence of electron transport in the succinate dehydrogenase reaction mechanism is: A) Coenzyme Q (UQ) B) FAD C) iron-sulfur clusters D) electron transport chain
B) FAD C) Iron-sulfur clusters A) Coenzyme Q D) ETC
Which of the following statements regarding the glyoxylate cycle is true?
two molecules of acetyl CoA can be converted to one molecule of oxaloacetate
What are the principal allosteric regulatory "signals" controlling the TCA cycle activity
acetyl-CoA NADH NAD+ ATP
Mammalian pyruvate dehydrogenase is regulated by phosphorylation using the enzyme__ which is allosterically activated by high levels of __ and __.
pyruvate dehydrogenase kinase; NADH; acetyl-CoA
The isocitrate lyase catalyzed reaction cleaves isocitrate into
succinate and glyoxylate
What is an inhibitor of complex IV?
cyanide
The malate-aspartate shuttle transfers ___ from cytosol to matrix while ____ moves from matrix to cytosol
malate; a-ketoglutarate
Where does the energy that drives ATP synthesis come from?
the proton gradient
the reaction of photosynthesis whereby light energy is transduced into chemical energy uses ___as a source of carbon; and produces ___
CO2; hexose
light-dependent electron flow with the production of ATP can occur without the formation of NADPH. This process is called ___, which involves only one of the photosystems, ____.
cyclic photophosphorylation; P700
Characteristics of chlorophyll include all EXCEPT:
iron-containing porphyrin
which of the following statements about the light reactions of photosynthesis is true? There are two distinct photosystems, linked together by a mechanism similar to electron transport. The source for electrons is located in the stroma of the thylakoid. The Mn complex is the primary el…
1) there are two distinct photosystems linked together by a mechanism similar to electron transport.
the light reactions in photosynthetic plants:
involve the splitting of two water molecules to produce molecular oxygen
how many photons of light must be absorbed by PSII for the oxidation of 2 water molecules to form one oxygen molecule?
4
All are properties of both the P700 and P680 reaction centers EXCEPT: contain chlorophyll dimers. are electron donors. are activated by visible light. are involved in light-driven O2 evolution. all are properties.
4) are involved in light-driven O2 evolution
The proton-motive force is composed of a ____ and a ____, and in photophosphorylation the ____ is greater.
membrane potential; pH gradient; pH gradient
Cyclic photophosphorylation takes place when
NADPH/NADP+ ratio is high, energy is used in generating ATP
light-dependent electron flow with the production of ATP can occur without the formation of NADPH. This process called __, which involves only one of the photosystem, ___.
cyclic photophosphorylation; p700
___and ___ are produced in the light reactions of photosynthesis which are used to convert __ to __
ATP; NADPH; carbon dioxide; sugar
Although the ratio of carboxylase to oxygenate activity of rubisco is about ___ TO ONE, the oxygenate activity diminishes plant productivity because of the loss of ___
3-4; ribulose-1,5-bisphosphate
The essential enzyme in C-4 plants used to conserve CO2 and avoid photosphorylation is:
PEP carboxylase

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