BIOCHEM 501: Practice Exam
35 Cards in this Set
| Front | Back |
|---|---|
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What is the nucleophile in DNA polymerase?
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3' OH
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WHat is the the nucleophile in DNA ligase?
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3'OH
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What is the nucleophile in RNA polymerase?
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3'OH
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What is the nucleophile in tyrosine recombinase?
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3'OH on the tyrosine
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Topoisoerase I nicks DNA using what nucleophile?
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tyrosine OH
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What does the DNA polymerase 3' to 5' exonuclease reognize?
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3' misincorporated nucleotides that fail to base pair with template
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Deamination of cytosine could lead to?
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CG to TA transition mutation
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Can RNA/DNA polymerase initiate polynucleotide synthesis de novo?
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RNA always can
DNA never can
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A change in the type of sigma factor will change what?
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The type of genes that are transcribed
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mRNA 3' end processing involves:
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protein recognition of RNA sequence
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mRNA intron removal uses what nucleophile?
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2'oh and 3'oh
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Mis charging tRNA with an incorrect amno acid can lead to what?
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incorperation of the wrong amno acid during protein synthesis
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What do miRNAs do?
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They down regulate expression of genes by cutting
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Are both tyrosine and serine recombinases using double stranded DNA?
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No
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What amino acids would most likely to be found on the surface of a protein?
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Asparagine, arginine, glutamine, lysine
(Positive charged)
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When do enzymes bind more tightly?
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Transition state
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What types of reverse inibitors of enzymes are there?
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Competitive, uncompetittive, mixed
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What does the michaelis menton equation describe?
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Hyperbolic function tlking about max velocity and K to talk about high tightly the enzyme binds
|
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Does a high or low Kcat/Km describe a catalytically efficient enzyme?
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High
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Primary active transport across a membrane occurs by
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Using ATP, a pump, a transporter
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What does a positive Delta G mean?
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the reaction is endergonic, the reaction is not favorable.
|
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What bonds can be oxidized/ broken for energy?
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C-H or C-C bonds
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Most of the oxygen we consume is turned into?
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Water
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What does uncoupling do?
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It prevents ATP formation
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WHere does the O2 made during photosynthesis come from?
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Water
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Is ubiquinone soluble in membranes?
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Yes
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What inhibits FA synthesis?
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palmitate
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What part of a lipoprotein is recognized by the LDL receptor?
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apolpoprotein
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Where is glycogen stored?
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mucle, brain, liver
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What is the purpose of glycogen in muscles and most other cells?
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short term fuel source for the generation of ATP
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What does insulin stimulate? Why?
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Glut4 to PM, FA synthesis Cholesterol synthesis, uptake of glucose
To lower blood sugar levels
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What is an insulin receptor?
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Enxyme and hormone binding protein
|
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How do steroid hormones transmit their signals?
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Ligand activated receptors
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What inhibits pentose phosphate pathway
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NADPH
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What does F2,6 BP do?
|
stimulates glycolysis (PFK1) and inhibits
gluconeogenisis (F 1,3 BP1)
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BIOCHEM 501: Practice Exam