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BIOL-L 211: DNA REPLICATION
Semiconservative replication
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method in which DNA replicates.
parent DNA splits into 2 daughter strands. 2 daughter strands add new strand
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Origin of replication |
many places where DNA unwinds creating relication forks
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Helicase |
the enzyme that unwinds the DNA helix generating single strand regions of DNA
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SSB
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single-strand binding protein
goes where helicase has been to keep the 2 strands from reconnecting
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DNA gyrase
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enzyme, topiosomerase
releives strand strain (supercoil)
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DNA polymerase |
A DNA polymerase is an enzyme that helps catalyze in the polymerization of deoxyribonucleotides into a DNA strand.
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Primase
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a RNA polymerase
primase-> generates RNA-> primer
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RNA Primer |
indicates to DNA polymerase where to start.
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DNA |
dounble stranded
sugar=deoxyribose
Bas paring: A/T, G/C
found in nucleus only
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RNA
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single stranded
sugar=ribose
base paring: A/U, G/C
found in nucleus and cytoplasm
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mRNA
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created in transiption
carries genetic message from nucles to cytoplasm
eukaryotes= monocistronic: mRNA trnaslates into only one product
prokaryotes= polycitronic: mRNA different proteins can be formed by starting different position on the mRNA
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tRNA
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transfer RNA
found in cytoplasm
links amino acids into the polypeptide chain.
different tRNA for each a.a
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rRNA
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ribosomal RNA
sunthesized in nucleolous
becomes intergral part of ribsomes complex
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hnRNA
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precursor to mRNA
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DNA ligase |
joins the okazaki fragments of teh lagging strand
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