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Biol 101: Exam 3

Eutrophication
1. Pollution- fertilizer 2. Algal Bloom- algae/autotrophs 3. Hetertrophs Bloom- consume all O2, photosynthesis and cellular respiration increase 4. O2 levels plummet 5. Dead Zones- low O2 conc in water
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Photosynthesis Equation
CO2+H2O-->Glucose+O2
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Autotrophs
Producers that make their own food
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Heterotrophs
Consumers that can't make their own food
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Types of organisms capable of photosynthesis
Autotrophs- plants, bacteria, and algae
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Chloroplasts
organelles consisting of photosynthetic pigments, enzymes, and other molecules grouped together in membranes major sites of photosynthesis
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Stomata
small openings between the two guard cells on the underside of leaf epidermis through which gas passes (CO2 in and O2 out)
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Chlorophyll
important light absorbing pigment in chloroplasts. responsible for green color green light reflected and all others absorbed
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cartonoids
absorb mainly blue-green light and reflect yellow, red, and orange. present all year temperature sensitive
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light reactions
products: electrons and ATP. These products go to dark reactions--makes glucose from CO2. CO2 comes from the atmosphere
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global warming
increasing concentrations of greenhouse gases. slow but steady rise in earth's surface temperature
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greenhouse effect
results from solar energy warming our planet. gases in atmosphere reflect heat bath to earth
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viruses
not living because the cannot reproduce on their own and don't have energy processing
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capsid
protein coat that DNA or RNA are enclosed in
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viral envelope
lipid bilayer membrane formed by a host cell
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lytic cycle
1. virus attaches to cell membrane 2. virus injects DNA into the cell 3. virus DNA takes over the cell telling it to replicate viral DNA and make new capsids 4. capsids and viral DNA assemble into new viruses 5. an enzyme for the virus breaks the cell membrane releasing new viruses
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Lysogenic cycle
1. phage attaches to host cell and injects DNA 2. phage DNA integrates into host cells genome like a plasmid 3. host cell can replicate 4. exposed to certain chemicals, and phage induction occurs 4. the prophage DNA is excised and enters the lytic cycle
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retrovirus
RNA virus that reverse transcribes its RNA to DNA once the RNA is in the host cell Goes backwards from what normal cells do
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reverse transcriptase
enzyme that the virus use to copy its RNA to DNA
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nucleotides
3 parts: nitrogenous bases, pentose sugar, phosphate groups DNA: adenine&thymine, cytosine&guanine RNA: adenine&uracil, cytosine&guanine
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semiconservative replication
new DNA molecules are 1/2 original and 1/2 new
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DNA polymerase
builds new DNA polymer
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DNA helicase
breaks H bonds and unwinds helix
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cell flow of information
DNA-->RNA-->Protein
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transcription
DNA gelicase opens/unwinds a specific gene between DNA and mRNA
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RNA polymerase
builds mRNA from a single template strand
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mutation
any change in DNA sequence that is inherited
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frame shift mutation
affects every codon past the mutation occur by insertions or deletions
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base substitution mutation
one nucleotide is substituted with another
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bacteriophage/phage
DNA virus
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genomic library
collection of all the cloned DNA fragments from a target genome
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genetic engineering
manipulating genes for practical purposes
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biotechnology
exploitation of biological processes for industrial and other purposes
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recombinant DNA
formed by joining DNA sequences from2 diff sources
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GMO's
contain one or more genes introduced by artificial means
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restriction enzymes
cut at specific DNA sequences (restriction sites)
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Staggered cut/sticky ends
when the last sequence is cut off and goes with the next sequence
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DNA ligase
enzyme that pastes DNA fragments together
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products of DNA technology and gene cloning
therapeutic hormones, diagnosis and treatment of disease, vaccines
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Concerns of GMO's
can introduce allergens into the food supply may spread genes to closely related organisms
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transgenic organisms
contain at least one gene from another organism
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gene therapy
aims to treat a disease by supplying a functional allele challenges: safe delivery to body area, not long-lasting, ethical questions
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DNA PROFILING
analysis of DNA fragments to determine whether they come from a particular individual
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Agarose gel electrophoresis
separates DNA molecules based on size
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STR analysis
used to compare DNA on one or more samples
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PCR
method of amplifying a specific segment of a DNA molecule. Sample is heated and cooled until sequence is copied
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genomics
study of an organisms complete set of genes and their interactions
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proteomics
studies the proteome, the complete set of proteins specified by a genome investigates protein functions and interactions
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plasmid
small circular DNA molecules independent of the bacterial chromosome. often used as vectors because they are a good source of gene carriers
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vaccines
stimulate an immune response by injecting proteins or infectious agents
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stem cells
immature cells that can divide and differentiate into specialized cell types
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Adult stem cells
stem cells located in tissues that help maintain and regenerate those tissues
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embryonic stem cells
come from embryos and can develop into almost any cell type
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