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MIT 7 014 - Biochemistry—Photosynthesis and Respiration

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Recitation Section 5 Answer KeyBiochemistry—Photosynthesis and RespirationRecitation Section 5 Answer Key February 27-28, 2006 Biochemistry—Photosynthesis and Respiration A. Photosynthesis background 1. Why do we consider O2 a booster of evolution? The organisms doing only glycolysis were slow and small. The net gain of 2ATP molecules from a molecule of glucose is not enough to fuel more complicated organisms that populate the biosphere today. For that, photosynthesis and respiration needed to develop. The waste product of non-cyclic photosynthesis is O2, and its presence allowed respiration to develop, since O2 could be used as an electron acceptor. 2. Why was it advantageous for a cell to develop photosynthesis? When abiotic sources of energy started to decline, it became advantageous to have the ability to make ATP. While glycolysis allows an organism to make ATP, it requires a source of organic carbon. Developing photosynthesis allowed an organism to become an autotroph—make everything it needs from CO2, NH3, PO4, H2O. Thus, in the environment low in abiotic sources of energy and carbohydrates photosynthetic organisms have selective advantage. 3. Photosynethesis has two phases—light and dark. What does each phase accomplish? The light phase is so named because it is dependent on the presence of light to drive its reactions. Light phase reactions use the energy of the excited chlorophylls to make ATP and NADPH, while producing O2 as a waste product. The dark phase is so named because it is independent from the presence of light. Dark phase is also known as a biosynthetic phase—the phase in which atmospheric CO2 is fixed into organic carbon. B. Photosynthesis—light reactions 1. Below are the schematics for cyclic and noncyclic photophosphorylation. Identify each schematic and discuss the order of steps in each process. +HPS1PS2 Electron acceptorElectron acceptor A2 Acceptor 3 Stroma Thylakoid membrane ATP synthaseH+H + H + H + H+H + ADP+ PiATP+H+H++HH+HFirst schematic is of cyclic photophosphorylation. Steps: 1) The process starts when a photon hits a PS1(photosystem 1) chloroplast, exciting two electrons on the molecule. 2) The two electrons are donated to the electron acceptor (creating a “hole” in the molecule), then 3) Passed to Acceptor 2(A2), and on to Acceptor 3 [both of these have names, but you do not need to remember them]. The later step powers the active transport of hydrogen ions from stroma into the thylakoid space. 4) Electrons pass from Acceptor 3 back to the PS1 chloroplast (thus, filling the “hole” in the molecule left by the electrons in step 2)). 5) H+ gradient drives the ions through the ATP synthase, powering the addition of a Pi to an ADP, creating ATP. Second schematic is of noncyclic photophosphorylation. Steps: 1) The process starts when a photon hits a PS2(photosystem 2) chloroplast, exciting two electrons on the molecule. 2) The two electrons are donated to the electron acceptor (creating a “hole” in the molecule). 3) Water is split in the thylakoid, providing the electrons to fill the “hole” in the PS2 molecule. O2 is released as waste product. 2H+ ions are created, to be used in step 7 below. 4) Electrons from the acceptor are passed to A2’, and on to Acceptor 3 [again, all of these have names, but you do not need to remember them]. The later step powers the active transport of hydrogen ions from stroma into the thylakoid space. 5) Electrons pass from Acceptor 3 to the PS1 chloroplast that was hit by a separate photon, exciting it, and creating a “hole.” 6) Electrons from the PS1 chloroplast are passed on to the electron acceptor and on to A2. 7) Electrons on A2 and the protons generated in step 3 are used to reduce NADP+ to NADPH+H+. 8) H+ gradient drives the ions through the ATP synthase, powering the addition of a Pi to an ADP, creating ATP. PS1PS2 Electron acceptorElectron acceptor A2 Acceptor 3 Stroma Thylakoid membrane ATP synthaseH+NADP+NADPH H+2H + H + H + H+H + ADP+ PiATPA2’ 2e +HH2O +H++½ (O2) HH+H2. What are the main differences between the two processes? In cyclic photophosphorylation, electrons come back to chloroplast molecules, while in noncyclic photophosphorylation, the splitting of water provides the electrons to “compensate” the original chloroplast, and produces O2 as waste product. In addition, noncyclic photophosphorylation produces NADPH as well as ATP. Cyclic photophosphorylation only produces ATP. 3. What is the ATP made in photosynthesis used for? It is mostly used in dark reactions of photosynthesis to fix CO2 into glucose. 4. In class we learned that cyclic photophosphorylation produces S as waste product. Yet we do not see S created anywhere in the schematic above. Where and how is S released? In order to fix CO2 into organic carbon, a lot of reducing power is needed. This power is provided by NADPH. In cyclic photophosphorylation as we described it so far no NADPH is produced. Another, side reaction is run to produce NADPH from NADP+. In that reaction, H2S serves as an electron donor, and S is released as waste product. 5. Why do cells need to make glucose out of ATP? Why not just use the ATP produced in photosynthesis? -Hard to store ATP (for long term storage, want currency in gold, not $) -Need glucose for other pathways. Intricate web of pathways—allows a non-photosynthetic cell (a heterotroph) to live on sugar source alone, or a photosynthetic cell (an autotroph) to build everything it needs from a small number of precursors. Many reactions along the way are endergonic—use ATP to power them. C. Aerobic Respiration – Oxidative Phosphorylation Dark reactions of photosynthesis are expensive, and glycolysis only produces 2 molecules of ATP. This is very inefficient. 1. What process developed to derive energy from glucose more efficiently? Why was it not possible to develop this process before noncyclic photophosphorylation arose? Oxidative phosphorylation, or respiratory chain, or electron transport chain. The process requires O2 as the final electron acceptor, so it could not have arisen before the advent of noncyclic photophosphorylation. 2. Why was it evolutionarily advantageous for a cell to develop aerobic respiration? An organism that can utilize its energy reserves more efficiently does not need to make or import from the environment as much glucose as a less efficient organism. Thus, in the


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MIT 7 014 - Biochemistry—Photosynthesis and Respiration

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