By adding SDS (sodium dodecyl sulfate)during the electrophoresis of proteins, itis possible to:A) determine whether a purified proteinconsists of multiple subunits.B) preserve a protein’s native structure andbiological activity.C) separate proteins exclusively on thebasis of molecular weight.D) A and C.E) A, B and C.• Protein Structure and Function in theImmune SystemFig. 15-1B and TIg (immunoglobulin) structureB and TFig 15-2Fig. 15-3B and TFig. 5-24LehnB and TFig 15-5Antibody diversity is generated first by gene rearrangementVLCLVHCH1Fig. 15-6B and TFig. 5-23LehnFig. 5-23LehnA constant domain of an Ig chain(antiparallel beta barrel)B and T15-7Fig. 15-8B and TComparison of the structures of a constant and of a variable domainWhich of the following parts of theIgG molecule are not involved inbinding to an antigen?A. FabB. FcC. Heavy chainD. Light chainE. Variable domainFig. 5-23LehnFig. 15-9B and TVariable and constant domains in Ig chainsB and T15-10Packing of the beta sheets from the constant domains of the heavyand light chainsThe VH and VL domains pack together differently than do the constant domains from the two chainsB and T15-11Like this Not like thisFig. 15-12B and TFour beta strands from each of the variable domains (VH and VL)each form half of a beta barrelB and T15-13Space-filling model of the hypervariable regions of an Fab fragmentComplex between lysozyme(antigen - in green) andantibody (blue and yellow)Regions of contact are red.B and T15-15Environment of Gln 121 (purple inprevious slide) in the lysozyme-antilysozyme complexB and T15-16Fig. 5-27LehnFig. 5-27LehnFig. 5-27LehnFig. 5-27LehnAntibodies can be used to probe forproteins that are only a small percentageof a complex mixture(immunoblotting)Antibodies can be used tovisualize specific types of cellsin a
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