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UIC BIOS 100 - Exam III

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-1-BIOS 100 - Summer, 2009 Name:Exam III, 5 August, 2009Michael Muller, Instructor TA:This exam consists of 45 questions. Each question is worth two points, and you’ll get 10 bonuspoints for bubbling in your name properly. Please check to see that all the pages are presentbefore you begin. Use a#2 pencil and bubble in all answers. Choose the best answer for eachquestion. Your score will be posted on the UIC Blackboard site as soon as they are in. GoodLuck!1. Predict the expression of a lacI S mutant. A lacI S mutant synthesizes a repressor protein thatcannot bind to the inducer lactose.A. The lac genes would be expressed frequently and efficiently only in the absence oflactose and presence of glucose.B. The lac genes would be expressed frequently and efficiently only in the presence of both lactose and glucose.C. The lac genes would be expressed continuously.D. The lac genes would never be expressed efficiently.E. Expression of the lac genes would be solely dependent upon the presence or absenceof glucose2. IPTG (Isopropyl â-D-1-thiogalactopyranoside) is a chemical which can bind to the repressorprotein of the lac operon but is not broken down by â-galactosidase (or any other enzymerelative to this problem). You have two E. coli cultures with fully functional lac operons. In one culture you add lactose and in the second you add an equivalent amount of IPTG.There is no glucose present in either culture. What would you expect to be the level ofgene expression in both cultures?A. Both cultures have continuous expression of the lac operon for a very long time.B. Both cultures have initial expression of the lac operon, but expression in the lactose-added culture ends while expression of the IPTG-added culture is continuous.C. Both cultures have initial expression of the lac operon, but expression in the IPTG-added culture ends while expression of the lactose-added culture is continuousD. Both cultures have initial expression of the lac operon, but expression in both culturessoon endsE. There is no expression in the IPTG-added culture3. Which of the following mutations will result in a lac operon in which expression is ONLYdependent upon lactose concentrations, not glucose concentrations.A. A mutant repressor protein which cannot bind to lactoseB. An mutant operator to which the repressor protein cannot bindC. A mutant repressor protein to which lactose permanently bindsD. A mutant promoter to which RNA polymerase easily bindsE. None of the above mutations produce a lac operon in which expression is ONLYdependent upon lactose concentrations, not glucose concentrations-2-4. The lac operon is an example of a(n) _________ operon. The trp operon is an example ofa(n) _________ operonA. inducible; inducible B. inducible; repressibleC. repressible; inducible D. repressible; repressible5. Which of the following is NOT necessary for proper expression of a eukaryotic geneA. Histone Acetyl Transferase (HAT) remodeling the chromatinB. TFIID binding to the basal promoter of the eukaryotic geneC. Removal of introns from the mRNAD. A transcription factor binding to a silencerE. All of the above are necessary for proper expression of an eukaryotic gene6. What would happen to a eukaryote if the gene which produces TATA-binding protein (TBP)were mutated so that the TBP could no longer bind to the basal promoter?A. The organism would quickly dieB. The organism would have about a 50% chance of livingC. The organism would live, but it would have reduced functionD. The mutation would not have a significant effect on the organismE. The organism would thrive with this mutation7. You discover a protein that you call PxcII. You discover that this protein has zinc fingers. What assumptions could you make about this protien?A. The protein binds to the basal promoter of eukaryotic genesB. The protein is a transcription factorC. The protein can bind to DNA, but you cannot assume any other functionD. The protein is involved in stabilizing mRNAE. You cannot make any assumptions about this proteinQuestions 8 to 9 all relate to a 2n = 8 cell8. The cell illustrated below is in what stage of cell division?A. Metaphase IB. Metaphase IIC. Anaphase ID. Anaphase IIE. Metaphase of Mitosis9. The cell illustrated below is in what stage of cell division?A. Metaphase IB. Metaphase IIC. Anaphase ID. Anaphase IIE. Anaphase of Mitosis-3-10. During which stage(s) of meiosis does crossing over occur?A. Prophase I B. Metaphase I C. Prophase II D. Metaphase IIE. A & C11. What is the product of meiosis?A. Two genetically identical cells B. Two genetically unique cellsC. Four genetically identical cells D. Four genetically unique cells12. Why does the frequency of non-disjunction increase exponentially with a woman’s age?A. Older women typically mate with older men - the problem is with the sperm, not theeggs B. Older women frequently do not get proper nutrition which affects spindle formationC. There is a limited supply of enzymes regulating meiosis, and when you get older,there are fewer in your system than when you were youngerD. All of a woman’s eggs are suspended in metaphase I during fetal development. Olderwomen have been exposed to more hazards and toxins, so mistakes in separationare more commonE. Let’s face it, everything goes to hell when you get older, so why should this be anydifferent?13. Which of the following mutations will cause an increase in cell division in eukaryotesI. Mutations which cause increased expression of proto-oncogenesII. Mutations which cause decreased expression of proto-oncogenesIII. Mutations which cause increased expression of tumor-suppression genesIV. Mutations which cause decreased expression of tumor-suppression genesA. I only B. I, III only c. IV only D. II, IV onlyE. I, IV only14. A cell lining the inside of an artery is knocked loose and floating in the blood. Why won’tthis cell start growing and form a tumor?A. The PDGFs (Platelet-Derived Growth Factors) prevent cell divisionB. Contact inhibition - the cell is not in contact with another cell, so this would preventcell growthC. Anchorage dependence - the cell is not in contact with a substratum, so this wouldprevent cell growthD. Both B & CE. A, B, and C15. Which of the following (A-D) is NOT a valid difference between plants and animals? If allare valid differences, then choose EA. Plants perform a different type of meiosis


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UIC BIOS 100 - Exam III

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