Lecture 6 Microbial cultivation and isolation BIO119 I. Isolating a pure culture A. Sample consideration 1. Environmental samples 2. Clinical samples 3. Microbial communities 4. Mixed populations B. What kind of tools to we need? 1. Incubators (shaking/non-shaking) 2. Petri dishes 3. Inoculating loop 4. Bunsen burner 5. Test tubes 6. Sterilization equipment 7. Sterile pipettes 8. Chemicals and water C. Streak plate procedures (Fig. 5.10) Produced with a Trial Version of PDF Annotator - www.PDFAnnotator.comLecture 6 Microbial cultivation and isolation BIO119 D. Spread plate procedures (Fig. 5.11A and 5.11B) E. Pour plate (Fig. 5.11C) F. Anaerobic methods (Fig. 5.12) G. Maintaining stock cultures (Fig. 5.13) Produced with a Trial Version of PDF Annotator - www.PDFAnnotator.comLecture 6 Microbial cultivation and isolation BIO119 II. Ways to isolate a pure culture by enrichment A. First figure out what type of organism you want to isolate. B. Design the growth medium. C. Define the strategy of isolation (see A above) D. Selective culturing on glucose (Fig. 5.14) III. Example #1: The hunt for a haloalkaliphilic archeaon that respires arsenate. A. What is arsenate respiration? B. Why look for this organism (hint: exobiology)? C. What did our team do? D. Were we successful? IV. Example #2: Isolation of a facultative anaerobic arsenate respiring bacterium A. Why did we look for this type of organism? B. Where did we look? C. Our success story. Produced with a Trial Version of PDF Annotator -
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