UCSC BIOLOGY 119 - Microbial cultivation and isolation (3 pages)

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Microbial cultivation and isolation



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Microbial cultivation and isolation

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Pages:
3
School:
University of California, Santa Cruz
Course:
Biology 119 - Microbiology

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Produced with a Trial Version of PDF Annotator www PDFAnnotator com Lecture 6 Microbial cultivation and isolation I Isolating a pure culture A Sample consideration 1 Environmental samples 2 Clinical samples 3 Microbial communities 4 Mixed populations B What kind of tools to we need 1 Incubators shaking non shaking 2 Petri dishes 3 Inoculating loop 4 Bunsen burner 5 Test tubes 6 Sterilization equipment 7 Sterile pipettes 8 Chemicals and water C Streak plate procedures Fig 5 10 BIO119 Produced with a Trial Version of PDF Annotator www PDFAnnotator com Lecture 6 Microbial cultivation and isolation BIO119 D Spread plate procedures Fig 5 11A and 5 11B E Pour plate Fig 5 11C F Anaerobic methods Fig 5 12 G Maintaining stock cultures Fig 5 13 Produced with a Trial Version of PDF Annotator www PDFAnnotator com Lecture 6 Microbial cultivation and isolation BIO119 II Ways to isolate a pure culture by enrichment A First figure out what type of organism you want to isolate B Design the growth medium C Define the strategy of isolation see A above D Selective culturing on glucose Fig 5 14 III Example 1 The hunt for a haloalkaliphilic archeaon that respires arsenate A What is arsenate respiration B Why look for this organism hint exobiology C What did our team do D Were we successful IV Example 2 Isolation of a facultative anaerobic arsenate respiring bacterium A Why did we look for this type of organism B Where did we look C Our success story



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