Biology 3A Laboratory Name: Cellular Respiration Worksheet Lab Section: DID YOU REMEMBER TO BRING YOUR USB THUMBDRIVE TO LAB TODAY?A. Aerobic respiration in germinating peas1. Upload the data from the Pasco Xplorer LX to Excel and graph the CO2 production data as afunction of time for the normal and the freeze/thawed peas. Add a trendline with the slope ofthe line equation and the R2 value for both types of peas. Calculate the slope of each lineand enter them on Table 1. The slope of the line is the respiration rate (ml/min or ml•min-1).Calculate the mass-specific CO2 production.Table 1. Mass specific carbon dioxide production (ml/min/g) for normal and freeze/thawed peasTube Contents Slope (ml/min) Peas mass (g)Mass-specific CO2 production(ml/min/g or ml min-1g-1)1 Normal peas2 Freeze/Thaw2. State a reasonable hypothesis for this experiment.3. Using Excel, graph the mass specific CO2 production for the peas that would support yourhypothesis.4. Did freezing and then thawing the peas inhibit respiration? What was the percentage ofinhibition? Show your work below.5. Why was it necessary make the mass-specific determination?6. If you repeated the experiment with half as many seeds, how would the respiration rate(slope of the line) be different?BIO 3A Lab: Cellular Respiration (02/09) Page 1 of 37. Would the mass-specific respiration rate be different for the sample from question #6?Explain.B. CO2 production during aerobic respiration8. State a reasonable hypothesis being tested in this experiment.Table 2. Data for CO2 production during aerobic respirationTube Total mass of Organism (g)ml of NaOH Relative respirationrate (ml NaOH)Resp. Rate/ g of organism(ml NaOH/ g of organism)GoldfishElodeaControl ----- ----- -----9. Briefly explain why Elodea was placed in the dark.10.What most likely contributed to the observed differences in respiration rate betweenorganisms?11.Explain why you think goldfish were chosen for comparison.BIO 3A Lab: Cellular Respiration (02/09) Page 2 of 3C. Anaerobic respiration –Please record the 30 minute values on the computer for each fermentation tube.Table 3. Amount of carbon dioxide produced (measured in mm) as a result of anaerobic respiration in yeastTime (minutes)Tube Contents 0 5 10 15 20 25 301 DI H2O2 Sucrose3 Corn syrup12.Which is (are) the control tube(s)? Which are the experimental tube(s)?13.Smell the contents of the tube containing the most CO2. Describe this smell?14.Consider the results of this experiment. Use the 30-minute data from all the group results toanswer this question: Can yeast utilize all of the sugars equally well?a. State a reasonable hypothesis for the question aboveb. Run the appropriate statistical analysis for your hypothesisc. Construct an appropriately labeled graph for the results (do not forget to report thecalculated p-value and if it is a one- or two-tailed analysis)BIO 3A Lab: Cellular Respiration (02/09) Page 3 of