SDSU BIOL 557 - Syllabus (2 pages)

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Syllabus



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Syllabus

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Pages:
2
School:
San Diego State University
Course:
Biol 557 - Transmission Elec Mic Lab
Transmission Elec Mic Lab Documents

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BIOLOGY 557 ULTRASTRUCTURAL TECHNIQUES Fall 2009 Use of the Facility is predicated on successful completion of a portfolio of images utilizing these techniques and instruments All images generated as part of this class are the property of the EM Facility This laboratory is intended to train students in electron and light microscopic techniques and will take a minimum average of 8 hours per week in the laboratory Students are not permitted to work in the lab outside of 8 30 am 5 p m M F without express permission of Dr Barlow Students will learn how to prepare grids to support samples in the TEM preserve and embed samples for slicing create surface replicas of samples align and use the light and transmission electron microscope negatively stain small samples prepare knives used in slicing record and process images slice both thick and thin slices for light and electron microscopic examination Text ELECTRON MICROSCOPY J Bozzola and L Russell 2nd edition Jones Bartlett 1999 EM Facility instrument tutorials GRADING Late assignments incur a grading penalty Lab report I Negative staining 20 Friday noon October 23 Lab report II Thick sections 20 Friday noon October 30 Lab report III Thin slicing 20 Monday noon November 30 Lab report IV Shadowing 20 Friday noon December 4 Lab report V Class presentation 10 Tuesday class December 8 Lab report VI Tomography 10 Friday noon December 11 Lab Practicals TEM solo exam Microscopes cameras Blocks and thick sections Credit Credit Credit No later than Oct 2 No later than Oct 16 No later than Oct 16 Lab activities will consist primarily of labeled micrographs highlighting each of the techniques discussed Additional guidelines will be given out in class Lab work will be graded on attendance quality timeliness and adherence to guidelines Tentative schedule Week 1 9 1 Week 2 9 8 Week 3 9 15 Week 4 9 22 Week 5 9 29 Introduction to laboratory Grids negative stain TEM operation Watch grid loading insertion neg stain grids videos TEM operation



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