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Mizzou BIO_SC 1010 - BIO1010 FS14 L18-GMOs for Bb

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Welcome to General Principles and Concepts of Biology!Today’s Goals:Next Time…DiscussVI. What is a GMO?Example: Milk cows and recombinant Bovine Growth Hormone (rBGH)Slide 7Slide 8Slide 9Slide 10Slide 11Slide 12Slide 13Slide 14Slide 15Slide 16Slide 17Slide 18U3 IC1Slide 20Slide 21Slide 22Slide 23Slide 24Slide 25Slide 26Slide 27Slide 28Slide 29Slide 30Slide 31Slide 32Slide 33Slide 34Welcome to General Principles and Concepts of Biology!Bio Sci 1010, Sec. 2ANNOUNCEMENTS:Exam #2 multiple choice score posted (out of 90)Mastering Biology Assignment Due tonightSeminar assignmentDon’t wait!Due Nov. 21stToday’s Goals:Readings: Chapter 9, section 9.2-9.3IV. What is a GMO? A. Recombinant DNA technologyB. Types of GMOsC. ControversyNext Time…Readings: Chapter 10I. What is biological evolutionII. Natural selectionA. SelectionB. TermsC. Darwin’s Four PostulatesDiscussWhat does this mean to you as a consumer?VI. What is a GMO?A. Recombinant DNA technologyGenetic engineering permits precise control of gene expression in different circumstances© 2013 Pearson Education, Inc.Example: Milk cows and recombinant Bovine Growth Hormone (rBGH)About 1/3 of cows in the US are injected with rBGH which increases milk volume from cows about 20%.The same principles apply to other proteins.Clotting proteins for hemophiliacsInsulin for diabeticsFDA approval is needed for any new food that is not generally recognized as safe (GRAS).VI. What is a GMO?A. Recombinant DNA technology1. Combine two sequences of DNA that don’t exist in nature (place gene in a new genome).VI. What is a GMO?A. Recombinant DNA Technology2. Steps:a. Remove gene from chromosome using restriction enzymes.VI. What is a GMO?A. Recombinant DNA Technology2. Steps:a. Remove gene from chromosome using restriction enzymes.i. Cuts DNA at specific locationsVI. What is a GMO?2. Steps:a. Remove gene from chromosome using restriction enzymes.i. Cuts DNA at specific locationsii. Staggered pattern leaves “sticky ends”VI. What is a GMO?2. Steps:a. Remove gene from chromosome using restriction enzymes.i. Cuts DNA at specific locationsii. Staggered pattern leaves “sticky ends”iii. Unpaired bases form bonds with complementary sequenceVI. What is a GMO?A. Recombinant DNA Technology2. Steps:b. Use same restriction enzyme to cut plasmid.VI. What is a GMO?A. Recombinant DNA Technology2. Steps:b. Use same restriction enzyme to cut plasmid.i. Circular piece of DNA from bacteriaii. Same sticky endsVI. What is a GMO?A. Recombinant DNA Technology2. Steps:c. Fuse gene and plasmid = recombinant DNAA. Recombinant DNA Technology2. Steps:d. Insert recombinant DNA into bacterial cell.VI. What is a GMO?A. Recombinant DNA Technology2. Steps:d. Insert recombinant DNA into bacterial celli. Bacteria replicatesii. Express the new gene!VI. What is a GMO?A. Recombinant DNA Technology2. Steps3. Challengea. How to put new genetic material in the transgenic organism?VI. What is a GMO?A. Recombinant DNA Technology2. Steps3. Challengea. How to put new genetic material in the transgenic organism?b. Use pathogenic (disease-causing) organisms that can already enter cells!i. Ti (bacterial) plasmid in plantsii. Viruses in animal/humansVI. What is a GMO?U3 IC1U3 IC1Name 1 Student #1 Signature 1Name 2 Student #2 Signature 21.What is GM food?2.What is one concern about foods containing genetically modified plants?3.What is one myth about foods containing genetically modified plants?Keep your card - turn it in at the end of the hour!VI. What is a GMO?2008 Nobel Prize in ChemistryB. Genetically modified organisms (GMOs)1. Created using recombinant DNA2. BacteriaVI. What is a GMO?B. Genetically modified organisms (GMOs)1. Created using recombinant DNA2. Bacteriaa. Bioremediation: insert genes to make oil-eating enzymes.VI. What is a GMO?B. Genetically modified organisms (GMOs)2. Bacteriaa. Bioremediationb. Medical uses:VI. What is a GMO?B. Genetically modified organisms (GMOs)2. Bacteriaa. Bioremediationb. Medical uses:i. Insert genes for making hormones, proteins and vaccines (ex: insulin, clotting factors)VI. What is a GMO?B. Genetically modified organisms (GMOs)2. Bacteriaa. Bioremediationb. Medical uses:i. Insert genes for making hormones, proteins and vaccines (ex: insulin, clotting factors)ii. Purer and cheaper!VI. What is a GMO?B. Genetically modified organisms (GMOs)2. Bacteria3. PlantsVI. What is a GMO?B. Genetically modified organisms (GMOs)3. Crop plantsa. Increase shelf lifeExample: Flavr Savr slow ripening tomatoes taste better & waste less (no GM tomatoes currently on market)VI. What is a GMO?B. Genetically modified organisms (GMOs)3. Crop plantsa. Increase shelf lifeb. Increase yield VI. What is a GMO?B. Genetically modified organisms (GMOs)3. Crop plantsa. Increase shelf lifeb. Increase yield i. Freeze resistantVI. What is a GMO?VI. What is a GMO?Inserted cold tolerance gene from flounder fish into tomatoes!B. Genetically modified organisms (GMOs)3. Crop plantsa. Increase shelf lifeb. Increase yield i. Freeze resistantii. Drought tolerantExample: Monsanto DroughtGard corn.VI. What is a GMO?B. Genetically modified organisms (GMOs)3. Crop plantsb. Increase yield i. Freeze resistantii. Drought tolerantiii. Pest resistantExample: Toxins produced by Bacillus thuringiensis (or Bt) used as natural insecticide in plants.VI. What is a GMO?Genetically Not geneticallyModified ModifiedVI. What is a GMO?B. Genetically modified organisms (GMOs)3. Crop plantsb. Increase yield i. Freeze resistantii. Drought tolerantiii. Pest resistantiv. Herbicide resistanceExample: Roundup Ready soybeansVI. What is a


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Mizzou BIO_SC 1010 - BIO1010 FS14 L18-GMOs for Bb

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