BIOL 640 1st Edition Lecture 15 Outline of Last Lecture I. Cellular Immunology Outline of Current Lecture II.Chromium release assayIII. Fluorescence activated cell sorter (FACS)IV. Paramagnetic behaviorCurrent LectureImmunology lecture 16: Lymphocytes:Tools for identifying cells:Development of CD antigens: clusters of differentiation:Molecules on cell surface are characteristics of cells or finger prints of cells.Chromium release assay:To measure the CD cells in a population, radioactive chromium is added which binds tightly to cells. The percentage of cpm in supernatant is measure.Fluorescence activated cell sorter (FACS):This can sort cells or quantitatively measure. 2 colors are used: red and green.If cells are charged with required levels of fluorescence, cells flow to that side and are collected in tubes.If cells have no charge, they flow to the tubes for low fluorescence emission.2D graph plot is drawn for analysis. Red- y-axis and green x-axis.These notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.Paramagnetic behavior:Magnetic properties are present only in magnetic field and therefore can not be magnetized. Strong paramagnet pulls cells to one
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