MIC 230 1st Edition Lecture 9 Outline of Last Lecture I. Cytoplasma. Inclusion Bodiesb. Polyphosphatec. Sulfur Granules II. EndosporesOutline of Current Lecture III. Metabolisma. CatabolismIV. Anabolism V. Nutrient ClassificationsVI. Nutrient RequirementsVII. Growth Current LectureNutrition and Growth*Cells have one goalreproduce*1. Metabolismsum of all biochemical reactions that occur in the cella. Catabolismgeneral energy-Breakdown of a chemical to generate energy (in broader sense—use of chemicalor light)-Breaking down larger chemicals to simpler-Either released to waste or used by cell for biosynthesis2. Anabolismbiosynthesis of cell componentsa. 3 requirements for anabolismi. Energy (typically) [from catabolism]ii. Substrates/nutrients/building blocks1. From environments2. From metabolism/catabolism iii. Reducing power (e-) [from catabolism]3. Nutrient classificationsa. Look at the bottom graph on the handoutThese notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.4. Nutrient requirementsa. H20b. Essential nutrients: CHONPS and Se (Fig. 4.1 in text)c. Trace elements/nutrients-Fe, Mg, Ca, Na, K…so on d. Oxygen- Classified based on O2 requirement (This list is based off of a, b, c, d, and e from Fig. 5.26 in text; also on handout)i. Strict or obligate aerobeii. Strict of obligate aerobeiii. Facultative anaerobecan grow using O2 or grow without O2iv. Microaerophilerequires O2 BUT at concentrations less than atmospherev. Aerotolerantcan grow in the presence of O2, but doesn’t use it and can grow without it5. Growth-Not growth of individual, but of population # of cells or amount of biomass of populationa. Cell divisioni. Most do binary fissionii. Buddingiii. Fragmentation iv. Reproductive spores NOT ENDOSPORES b. Growth Curve-Time for the population to double=generation time or doubling time -Growth rateincrease in cells over time (Fig. 5.8…doubling time is half an hour)c. Continuous Culture (Fig. 5.11 in text)i. Chemostat:-Continuous out flow (effluent)-Removes cell (no overcrowding)-Removes
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