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UWL MIC 230 - Energy and Nutrient Requirements for Cells

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MIC 230 1st Edition Lecture 9 Outline of Last Lecture I. Cytoplasma. Inclusion Bodiesb. Polyphosphatec. Sulfur Granules II. EndosporesOutline of Current Lecture III. Metabolisma. CatabolismIV. Anabolism V. Nutrient ClassificationsVI. Nutrient RequirementsVII. Growth Current LectureNutrition and Growth*Cells have one goalreproduce*1. Metabolismsum of all biochemical reactions that occur in the cella. Catabolismgeneral energy-Breakdown of a chemical to generate energy (in broader sense—use of chemicalor light)-Breaking down larger chemicals to simpler-Either released to waste or used by cell for biosynthesis2. Anabolismbiosynthesis of cell componentsa. 3 requirements for anabolismi. Energy (typically) [from catabolism]ii. Substrates/nutrients/building blocks1. From environments2. From metabolism/catabolism iii. Reducing power (e-) [from catabolism]3. Nutrient classificationsa. Look at the bottom graph on the handoutThese notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.4. Nutrient requirementsa. H20b. Essential nutrients: CHONPS and Se (Fig. 4.1 in text)c. Trace elements/nutrients-Fe, Mg, Ca, Na, K…so on d. Oxygen- Classified based on O2 requirement (This list is based off of a, b, c, d, and e from Fig. 5.26 in text; also on handout)i. Strict or obligate aerobeii. Strict of obligate aerobeiii. Facultative anaerobecan grow using O2 or grow without O2iv. Microaerophilerequires O2 BUT at concentrations less than atmospherev. Aerotolerantcan grow in the presence of O2, but doesn’t use it and can grow without it5. Growth-Not growth of individual, but of population # of cells or amount of biomass of populationa. Cell divisioni. Most do binary fissionii. Buddingiii. Fragmentation iv. Reproductive spores NOT ENDOSPORES b. Growth Curve-Time for the population to double=generation time or doubling time -Growth rateincrease in cells over time (Fig. 5.8…doubling time is half an hour)c. Continuous Culture (Fig. 5.11 in text)i. Chemostat:-Continuous out flow (effluent)-Removes cell (no overcrowding)-Removes


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UWL MIC 230 - Energy and Nutrient Requirements for Cells

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