BIOL 640 1st Edition Lecture 14Outline of Last Lecture I. RIA and ELISA Outline of Current Lecture II.Special ELISAIII. Western blottingIV. Cellular ImmunologyCurrent LectureImmunology Lecture 15- Special ELISA:“ DIP STICK” used to detect early stages of pregnancy for the presence of human chrionic gonadotropin.It is mass produces and cheaper.There is capillart action of liquid. Over excess of Ab in R region and continues to C zone (attached Ab). T zone has attached Ab. Stripes are made on T and C zone are the results.Control zone after T zone , if reaction is positive at C zone, all reagents are biologically active.The HCG hormone is a small peptide molecule which in the tap water causes early ovulation in young girls. DHEC testing HIV:Plastic bead has Ag on surface. After washing, add goat anti (human Ig G). Wash and add substrate, where substrate to product is the end reaction.Plastic bead size and density is increased for more detection.Western Blotting:Electro blotting is done.These notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.Cross reactivity to same size of Ag. Ag of right size is detected. Ag Ab recognized.1977 Southern- DNA gel to membrane. Northern- RNA blotting. Western- Protein blotting.Cellular Immunology:It describes the system facing Ag.Lymphocyte -- lymph node --- blood.Resting mature lymphocytes have no secretions.Lymph node -- Subclavian vein --- back to circulation.Lymphocytes detect all cells except immuno priviledged sites.Immuno surveillance of blood done by spleen. There is only outgoing lymphatic from spleen.1 particular lymphocytes go through all levels of cycle.MALT Mucosal associated lymph tissues.SALT Skin associated lymph tissues.Lymph nodes leave blood vessel and is called extravasation, also at inflammation site.B cells in lymph nodes get activated and make Ab that enter blood stream through lymphatics.T cells at inflammation site fight infections.Enlarged lymph nodes are a sign of
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