Name: Bhavi PatelPartners: Subhrangi Swain and Jessica GreenTitle: How Old is the Chick Embryo?Date of Experiment: 09/22/2011Due Date: 9/28/11Experiment #1Abstract: In this lab, I dissected a chicken embryo so that I could take pictures of the embryo to estimate the age and the stage of the embryo. I hypothesized that I would be able to estimate the age of the embryo using a microscope and by taking pictures of the embryo. In this lab, I used tools to crack open the egg, then I observed the embryo under a microscope, I dissected the embryo, I dyed it and I took pictures of the embryo using a microscope with a camera before andafter it was dissected and dyed. From the pictures that I took, I estimated that my embryo was in its 17th + stage and 64 + hours old using the Hamburger Hamilton Staging Guide. Introduction: In biological sciences developing chick embryos have been used for a long duration of time because “their domestication as poultry makes them more readily available than other vertebrates (such as mice), and being oviparous, the embryos are easily accessible” (wiki). Therefore by using the chick embryo, students are able to see developing complex structures within the embryo that are common among other vertebrates. In addition to this, students are ableto use the Hamburger-Hamilton stages, “which are a series of 46 chronological stages in chick development, starting from laying of the egg and ending with a newly hatched chick,” (wiki) to estimate the age and the stage of the embryo. I hypothesized that I would be able to estimate the age and stage of my chick embryo using the Hamburger Hamilton Stages and the images I took of the embryo. I predict that I will be able to estimate the age and stage of my embryo using the pictures that I took using the microscope with a camera and the Hamburger Hamilton Stages.Methods: First I cracked the egg by using a probe and tapping repeatedly, after doing this I poured all the substance from inside the egg into a white container. Then I observed the embryo and saw the liquid surround the embryo, red lines coming out, and the embryo’s beating heart. After this I put the ringer solution, which is a salty and warm solution, into a dish to mimic its original environment, I took a spoon and took the embryo out of the container and put it into the dish. Then I took the excess yoke and liquid surrounding the embryo out using a pipette and next I observed the dish with the embryo under the microscope (15x) and I saw the beating heart and the outline of the embryo. After this I fixed the lighting of a microscope and took pictures using amicroscope with a camera. Afterwards, I used a probe and forceps to dissect the embryo and added more of the ringer solution, and once again looked at the embryo under the microscope (25x), and then I fixed the lighting of the microscope and took pictures of the dissected embryo using a microscope with a camera. Following this, I added two drops of purple dye into the dish with the embryo, the dye spread around the embryo, and then I fixed the lighting of the microscope and I took pictures of the embryo with the dye using a microscope with a camera. Next, I observed the embryo under the microscope (30x). After this, I added more dye to the embryo so I can see the structures of the embryo even better and fixed the lighting of the microscope and took more pictures of the embryo using the microscope with a camera. I then used the Hamburger Hamilton stages and compared my pictures with the different development stages on there to figure out an estimation of the age and stage my embryo was in.Results: I estimated that it was in its 17th stage which is a time period of development of about 72 hours, according to the Hamburger Hamilton Stages; I came to this conclusion by using the various pictures that I took of the embryo and by using my observations of it. I then used the Hamilton stages and compared my pictures with the different development stages on there to figure out an estimation of the age and stage my embryo was in. Below I have included one of the pictures I took of my embryo with the embryo’s developing structures labeled.Discussion: In the end, I estimated that my embryo was in its 17th stage which is a time period of development of about 72 hours, according to the Hamburger Hamilton Stages by using the various pictures that I took of the embryo. In addition to this, I came to this conclusion because I was able to see the heart beating, the eye, the mid brain, the olfactory pit, the anterior limb bud, the neural tube, and the somite in the embryo which lead to me to my conclusion because all of these are seen in the image shown on the Hamburger Hamilton Stage Guide. References: https://bcrc.bio.umass.edu/intro/sites/bcrc.bio.umass.edu.intro/files/chickstagingseries-1.pdfSource As stated on website: After Hamburger, V., and H. L. Hamilton, 1951. A series of normal stages in the development of the chick embryo. J. Morphol. 88: 49–92.http://en.wikipedia.org/wiki/Hamburger-Hamilton_stagesWikipedia, the free encyclopedia (12 August 2011), Hamburger-Hamilton stageshttp://www.devbio.net/node/55Source as stated on website: Chick staging based on Hamburger V and Hamilton, HL. 1951. A series of normal stages of development of the chick embryo. J Morph