MIC 230 1st Edition Lecture 6Outline of Last Lecture I. Differences between Bacteria and Archaea cell membranesII. Gram – vs. gram + cell wallsIII. PeptidoglycanOutline of Current Lecture IV. Gram Positive and Negative Cell Wall V. Outside of the Cell WallVI. Cell Wall Comparison Chart VII.Motility using Flagella Current Lecture3. Structure—Domain Bacteriaa. Peptidogylcanb. Gram-positive cell walli. Thick layer of PCii. Teichoic acids/lipoteichoic acidsc. Gram-negative cell walli. Thin layer of peptidoglycan ii. Outer membrane- asymmetric compared to CM, although has phospholipids outer layer of bilayer contains lipopolysaccharides (lipids and sugars)iii. Periplasm goes from CM to OMiv. Within outer membrane have porins to allow molecules through OMSide note: (phospholipids are a barrier)4. Structure—Domain Archaeaa. NONE have PGb. Protein, glycoprotein, polysaccharide, a FEW pseudopeptidoglycani. Lysozyme cannot cleave ii. Not affected by penicillin c. Protein or glycoprotein can form an s-layer (Fig. 3.22 in textbook)i. They are symmetrical, paracrystalline, it can be used as a cell wall which isunique to archaeaThese notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.OUTER LAYERS (back to previous lecture) outside the CWii. Slime Layer-loose- largely water iii. Capsule-ridged, doesn’t stain well (Fig. 3.23 in textbook)- largely wateriv. S-layer 5. Function a. adhesion i. Habitat stabilityii. Protection from phagocytosisb. Aids in slowing drying out Cell Wall Comparison ChartBacteriaGram +BacteriaGram -ArchaeaPeptidoglycan +Thick+Thin-Teichoic acid + - -Outer membrane _ + -Sensitive to penicillin + - -S-layer +Outside CW+Outside CW+As a CW or Outside CW3. Motility using Flagella (sing. flagellum)a. Structurei. Long proteinaceous filamentii. Semi-ridged- different from Euk. flagellaiii. “wavelength” b. Partsi. Basal BodyMotorii. Hook- which swivels/connectoriii. Filamentc. Arrangement of flagellai. Polar at an end1. mono (one) trich (hair) ous (pertaining to) = monotrichous2. lopho (tuft) trichous3. bilophitrichous/amphitrichous ii. Peritrichous (Around) (Fig. 3.44 in