ACC MLAB 1315 - EXERCISE 7: Automated Differential (11 pages)

Previewing pages 1, 2, 3, 4 of 11 page document View the full content.
View Full Document

EXERCISE 7: Automated Differential



Previewing pages 1, 2, 3, 4 of actual document.

View the full content.
View Full Document
View Full Document

EXERCISE 7: Automated Differential

16 views


Pages:
11
School:
Austin Community College District
Course:
Mlab 1315 - Hematology

Unformatted text preview:

EXERCISE 7 Automated Differential MLAB 1315 Hematology AUTOMATED DIFFERENTIAL LAB OBJECTIVE Dry lab 1 The student will interpret 5 differentials from the Coulter STKS the Bayer Advia and the Abbott Cell Dyne by examining the instrument printout of the CBC as well as the histograms and scattergrams 2 The student will state the theory of the technology used to perform the automated differential from 4 different instruments Wet lab The student will examine the scatterplots of CBCs performed at CPL and interpret the results stating whether the automated differentials are normal or abnormal PRINCIPLE Coulter STKS currently in use as a primary instrument at CPL and as a backup instrument at Seton Medical Center The Coulter STKS performs a five part differential consisting of monocyte lymphocyte granulocyte eosinophil and basophil The Coulter VCS established WBC differential technology uses three measurements individual cell volume high frequency conductivity and laser light scatter as explained below Volume Analysis Conductivity Scatter LAB EXERCISE 7 Electronic leukocyte volume analysis uses a low frequency current measure volume of the WBCs as they impede the current when they pass through the aperture Cell walls act as conductors to high frequency current As the current passes through he walls and through each cell interior it detects differences in the insulating properties of cell components It characterizes the nuclear and granular constituents and the chemical composition of the cell interior Leukocytes are hydrodynamically focused and passed in a steady stream through a sensing zone on which a laser light is focused As each cell passes through the sensing zone of the flow cell it scatters and reflects the focused light which is detected by a photodetector The patterns of scatter are measure at various angles forward scatter at 180 and right angle scatter at 90 Scattered light provides information about cell structure shape and reflectivity The characteristics are



View Full Document

Access the best Study Guides, Lecture Notes and Practice Exams

Loading Unlocking...
Login

Join to view EXERCISE 7: Automated Differential and access 3M+ class-specific study document.

or
We will never post anything without your permission.
Don't have an account?
Sign Up

Join to view EXERCISE 7: Automated Differential and access 3M+ class-specific study document.

or

By creating an account you agree to our Privacy Policy and Terms Of Use

Already a member?