Berkeley MCELLBI 110 - Exam Answers (10 pages)

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Exam Answers

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10
School:
University of California, Berkeley
Course:
Mcellbi 110 - Molecular Biology: MacRomolecular Synthesis and Cel...
Molecular Biology: MacRomolecular Synthesis and Cel... Documents

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MCB110 FINAL May 14 2001 Your name and student ID QUESTION POINTS 1 15 points 2 15 points 3 15 points 4 10 points 5 10 points 6 25 points 7 15 points 8 10 points 9 15 points 10 10 points 11 15 points 12 20 points 13 10 points 14 5 points 15 50 points 16 50 points TOTAL 300 points WARNING Your exam will be taken apart and each question graded separately Therefore if you do not put your name and ID on every page or if you write an answer for one question on the backside of a page for a different question you are in danger of irreversibly LOSING POINTS Name Student ID QUESTION 1 a You are growing bacteria at room temperature and at 4 C What type of differences would you expect in the lipid composition of the two colonies 5 At 4 C lipids will have shorter chains and more unsaturated bonds b Which will have a higher rate of spontaneous flip flop phosphatidic acid or phosphatidyl serine and why 5 Phosphatidic acid as the polar head is smaller and less charged and thus less energetically unfavorable to pass through the hydrophobic bilayer c Can you think of a reason why phosphatidyl inositol is more abundant in the inner leaflet of the plasma membrane 5 Because of the involvement of PI phosphate derivatives in signal transduction QUESTION 2 You have cloned a new integral membrane protein from erythrocyte plasma membrane Analysis of the sequence has given you the following hydropathy plot Mass spectroscopy of the purified protein indicates than residues near the C terminus are glycosylated Can you draw a diagram of the most likely secondary structure of this protein and its orientation in the membrane with respect to the outside cytosol 5 How can you visually determine if this protein is attached to focal adhesions Tip you have available antibodies against the C terminus of the protein You should know two possible answers 10 Attachment to focal adhesions FA will restrict the movement of the protein in the membrane One could use FRAP or video microscopy with gold labeled



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