CALTECH APH 162 - Syllabus (4 pages)

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Syllabus



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Syllabus

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Pages:
4
School:
California Institute of Technology
Course:
Aph 162 - Physical Biology Laboratory

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BE APh 162 Winter 2009 Syllabus Professor Rob Phillips TAs Dave Wu Damien Soghoian Arbel Tadmor Hannah Tuson Mondays 2 5 PM Tuesdays 7 10 PM in 040 Keck Week 1 Size of things Session 1 Logistics and microscopy fundamentals Introduction to the course Microscopy fundamentals optics demonstration K hler illumination Oil water air objectives demo CCD How it works Signal quantization and saturation Pixel resolution Micromanager Session 2 Size of things using microscopy Light phase fluorescent microscopy Good practices Techniques Making agarose pads Calibration of microscope using calibration slides Light phase microscopy E coli grown overnight in poor vs rich media Schaechter et al observation Look at an assortment of live bacteria and Eukaryotes Fluorescent microscopy YFP labeled phages attached to E coli FM dye stained cyanobacteria membranes Various pre stained slides Week 2 Rate of things Session 1 Rate of things using microscopy Light phase microscopy Movie of E coli growth Movie of yeast growth Beating of Chlamy cilia Dictyostelium Fluorescent microscopy Photobleaching of fluorescent E coli cells Session 2 Rate of things using spectrophotometry Theory Diauxic growth curve using different sugars Operons and operon regulation Order of magnitude estimation Spectrophotometry Beer Lambert law Demonstration on chlorophyll OD600 vs cfus Experimental Measure diauxic growth curve 1 3 Glucose Lactose 1 3 Glucose Arabinose 1 3 Glucose Sorbitol 1 3 Glucose Maltose Plate cells Week 3 4 DNA engineering Session 1 PCR Outline experiment PCR YFP insert PCR protocol Proper pipetting techniques Execute PCR Restriction digest What we are digesting and why How to predict cutting sites internet Vector NTI Project discussion Session 2 Restriction digests and gels PCR purification of insert Digestion Insert Plasmid Lambda Gel electrophoresis Cast gels Talk about gel electrophoresis Set up samples for gel Load gel PCR purification of digested insert Session 3 Ligation and transformation



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