MIC 230 1st Edition Lecture 3 Outline of Last Lecture I. Continuation of the History of Microbiology II. The Two Functions Life RequiresIII. Porphyrin RingOutline of Current LectureIV. Theory of EndosymbiosisV. Important Founders of MicrobiologyVI. TaxonomyVII. Classification of Prokaryotes Current LectureTheory of Endosymbiosis-At some point a nucleated cell engulfed another cell and lived together and over time the endosymbiote devolved to become an organelle (no longer an independent organism)Lines of Evidence:1. Mitochondria and chloroplasts have their own DNAcircular2. Mitochondria and chloroplasts have their own ribosomes 70’s not 80’s3. Mitochondria and chloroplasts have ribosomal RNA who’s sequence is closelyrelated to bacterial rRNARobert Hooke (1665)-Saw cellsAnton Von Leeuwenhoek (Father of Biology, 1860’s)-Great at grinding lenses-Saw and PUBLISHED bacteriaLouis Pasteur (1860’s)These notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.-Disproved spontaneous generation -Swan neck flask-Know the process of this experiment. There is a slide on it.Development of electron microscope (EM, 1950’s)-Lead to the prokaryotes vs. eukaryote delineation -Functional group: eukaryotes have a nucleus and organelles; Prokaryotes do notTaxonomy science of naming and classification/identification -Traditionally phenotypic-Phylogenybased on evolutionary relationships of natural history-Fossils-Molecularassumption is the evolution of molecule = evolution of organismCarl Woese proposed 3 domains of life: Bacteria, archaea, and eukarya-Prokaryotes (functional group) are bacteria and archaea (domain names)-Eukaryotes (functional group) are eukarya (domain name) Classification of Prokaryotes-Traditional taxonomyphenotypic-Binomial nomenclature 2 names-Genus and specific epithet=species name Morphology/Shape-Coccus/cocci (look circular)-Rod (bacillus/bacilli)-Spirillum/spiralla-Vibrio (look like a comma)-Spirochetehelical-very different from spirillum under EM-FilamentousArrangement-Singular-Pairs-Chains-Irregular