UT BIO 325L - Homework9Spring2014[KEY] (4 pages)

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Homework9Spring2014[KEY]



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Homework9Spring2014[KEY]

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Pages:
4
School:
University of Texas at Austin
Course:
Bio 325l - Laboratory Experience in Genetics

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Name Lab room day time TA name Genetically modified organisms You will work with your own GMO data 1 The procedure you followed for testing for GMO foods makes heavy use of controls In fact only one PCR reaction was not a control It also has the interesting outcome that DNA or primers may be used in common between both positive and negative controls making the identification of each type tricky a 1 point Identify all of the positive control PCR reactions Briefly defend your choices The key difference between positive and negative controls is that positive controls should give you a measurable response while negative controls should have the absence of the measurable feature So any reaction that was supposed to give a band would be a positive control regardless of the specific combinations of templates and primers They are GMO template DNA with GMO specific primers GMO template DNA with plant specific primers Test food DNA with plant specific primers Non GMO food DNA with plant specific primers b 1 points Identify all of the negative control PCR reactions Briefly defend your choices Same reasoning as above except the negative controls are those reactions that should not produce a band The only one is Non GMO food DNA with GMO specific primers 2 1 point What was your tested food Was it genetically modified If yes or no explain with regards to the controls how you reached that conclusion If your results could not be interpreted accurately explain why not Answers will vary The soybean tested positive for GMO last semester and the cornmeal is a known one as well but the others won t be known until they are actually tested Reasoning for a yes There were bands for all of the positive controls and no band for the negative control There was a band in the test food DNA with GMO specific primers and it corresponded to the band in the GMO template DNA with the GMO specific primers positive control Reasoning for a no There were bands for all of the positive controls and no band



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