UT BIO 325L - Homework3_Spring14[key] (3 pages)

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Homework3_Spring14[key]



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Homework3_Spring14[key]

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Pages:
3
School:
University of Texas at Austin
Course:
Bio 325l - Laboratory Experience in Genetics

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Name Lab room day time TA name Fly Pre lab Review Homework 2 and the Anatomy of Experiments worksheet if you lost yours it is posted on Blackboard Some of this will be reviewed in the 2 3 lecture as well Familiarity with patterns is of great value when determining how genes are inherited using phenotypic data The following questions will help you think about those patterns and what you should see in the F1 generation in lab Remember You crossed true breeding i e homozygous or hemizygous white eyed male flies to virgin homozygous wild type females 1 2 points As you should have discovered from your previous homework assignment your white eyed flies could be caused by the singular white w mutation Write out the genotypes of the parental generation followed by the expected genotypes phenotypes and ratio of phenotypic classes seen in the F1 generation if the white eyed phenotype was caused by this white mutation They should have determined that white is recessive and X linked P X X x Xw Y Can also include the rest of the chromosomes Can also show as x w Y X Xw normal eye phenotype X Y normal eye phenotype Note that order matters Female contributions are shown first on top so Xw X would be inaccurate Ratio of phenotypes determined from a punnet square is going to be 2 wild type females 2 wild type males or 1 1 or if they don t count sex as a phenotypic class 100 are wild type 1 wild type 0 white eyed F1 2 Recall that null hypotheses help us define our baselines or controls Because of this you are going to have a somewhat counterintuitive null hypothesis that states what you think the pattern of inheritance is not what you think it is not This will make more sense when we get into the statistics of how you will test your null hypothesis a 1 point Write the null hypothesis for the white mutation Your null hypothesis needs to include the expected means of inheritance meaning it must reference if the mutation is dominant or recessive and autosomal or sex linked For more than



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