SC BIOL 620 - Complement (5 pages)

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BIOL 460 1st Edition Lecture 8 Outline of Last Lecture I Nucleotide Biosynthesis Outline of Current Lecture II Complement III Functions IV Nomenclature Current Lecture Immunology Lecture 9 Complement and its activation Sheep RBC immunized mouse alpha SRBC anti serum sheep RBC Alpha SRBC SRBC EXPECT agglutination OBSERVE SRBC lysis Ehrilch discovered that cellular cells Ag added to serum should show interaction of Ab Ag to form a lattice i e agglutination But instead SRBC lysis seen 50 degrees celcius 1 hr Alpha SRBC SRBC AGGLUTINATION Inference Substance causing lyses is not present after heat treatment 50 degrees celcius 1 hr Alpha SRBC normal mouse serum SRBC lysis These notes represent a detailed interpretation of the professor s lecture GradeBuddy is best used as a supplement to your own notes not as a substitute Normal serum has substance such that when the anti serum is added to SRBC causes lyses Learnt about the substance can be heat de activated exists in normal serum Triggered by Ag Ab recognition Control experiment Normal serum SRBC NOTHING A complement where heat reacted anti serum with the serum causes lyses SRBC Inactive complement active complement lyse SRBC Complement is about 20 proteins made by the liver similar system is found in invertebrates and plants Functions 1 2 3 4 Cell lysis Helps in opsonization via phagocytosis binding Activation of inflammatory response by anaphylatoxins C3a C5a Clearance of immuno complexes from blood Reaction kinetics of a cascade A A Active A Active B B Active B Active C C Active C Active Same kinetics 1 sec convert 100 molecules in each step In 3 sec 100 x 100 x100 10 6 molecules D D Active Cascade Major amplification Simple reaction without cascade A Active D D Active In 3 secs only 300 D Active molecules Complement Activation 1 Covalent Modifications make or break covalent bonds 2 Conformational change of a protein Nomenclature Classical Pathway C1 C9 Lectin Pathway Mannose binding Lectin Alternate Pathway B D P