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CSU BMS 300 - DNA Replication to RNA Structure

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BMS 300 J. Walrond Lecture 8 Outline of Last Lecture I. Filamentous actin in the cortical cytoskeleton-myosin motors 1. 2. movement in F-actin -vesicle access to the membranes1. local delivery II. DNA structure, replication, and the central dogma -DNA as molecules of life 1. stores information about RNA structures 2. RNA structure stores information about protein structure III. DNA components -5carbon sugar dextrose -bases 1. purines >adenine>quinine 2. pyrimidines >thymine These notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.>cytosine IV. Polymerization of sugars -phosphodiester bonds V. Antiparallel structure and hydrogen bonds -role of bases VI. Replication Outline of Current Lecture I. DNA structure -phosphodiester bonds -antiparallel organization-hydrogen bonds II. DNA replication -semiconservative replication 1. each strand is a template for a new strand -role of helicase 1. unwind helix2. break hydrogen bonds -leading strand (DNA polymerase) 1. polymerase reads 3’ to 5’2. generates 5’ to 3’ III. The lagging strand -read 3’ to 5’IV. Components of RNA -ribose -bases1. purines >adenine >guanine 2. pyrimidines >uracil >cytosine(thymine is 5 methyl uracil) V. RNA polymerase -reads DNA 3’ to 5’ to make RNA 5’ to 3’ -transcription Current LectureDNA Structure-DNA is a double stranded helix >2 complimentary strands -It depends on the hydrogen bonding between purines and pyrimidines-Purines: >adenine>guanine-Pyrimidines:>cytosine>thymine Antiparallel organization of the DNA structure: opposite-when one strand is 3’ to 5’ and the complimentary strand is 5’ to 3’ -both strands are combined with a phosphodiester bond -both strands are linked together by hydrogen bondsDNA replication-semiconservative fashion >each old strand serves as a template for a new strand DNA polymerase: polymerizes phosphodiester bonds between adjacent deoxyribose >links 5’ to 3’ >”selects complementary base”RNA polymerase -matches base pairs then creates a phosphodiester bond -transcription: when the RNA always reads 3’ to 5’-the “TATA” box is where the transcription factor is -which leads to RNA editing: splicing (which we will go over in the next


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CSU BMS 300 - DNA Replication to RNA Structure

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