MEDICAL LAB TECHNOLOGY Glucose Test Blood sugar Random 1 Take 500 ul Reagent 2 Add 5 ul Sample Serum 3 Wait for 5 minutes and note your readings 4 Normal Values Random 70 160 Fasting 60 110 Heamoglobin Hb 1 Take 2500 ul Hb Reagent 2 Add 20 ul EDTA Blood 3 Wait for 10 minutes and note your readings 4 Normal Values Male 13 18 Female 8 14 Uric Acid 1 Take 500 ul Reagent 2 Add 10 ul Sample Serum 3 Make sure the blood will be clotted 4 Wait for 5 minutes and note your readings 5 Normal Values Male 3 4 7 0 Female 2 4 5 7 Renal Function Test RFTs Includes Urea Creatinine Bun Urea 1 Take 400 ul Reagent 1 2 Add 100 ul Reagent 2 3 Add 10 ul Sample Serum 4 Make sure the temp of Reagent must be at least 37 C 5 Read immediately Creatinine 1 Take 400 ul of Reagent 1 2 Add 100 ul of Reagent 2 3 After 3 to 4 minutes Add 50 ul Sample Serum 4 Read immediately and note your readings BUN 1 Bun can be calculated by dividing the urea s result by 2 4 2 E g Urea 36 BUN 15 36 2 4 Calcium 1 Take 250 ul Reagent 1 2 Add 250 ul Reagent 2 3 Add 10 ul of Sample Serum 4 Wait for 5 minutes and note your readings Liver Function Test LFT s Includes ALT SGPT AST SGOT ALP AFP Total Proteins Serum Albumin Bilirubin Direct Bilirubin Indirect ALT SGPT 1 Take 400 ul Reagent 1 2 Add 100 ul Reagent 2 3 After 3 4 minutes add 50 ul of Sample Serum 4 Read immediately note your readings 5 Normal Values 10 50 0 45 AST SGOT 1 Take 400 ul Reagent 1 2 Add 100 ul Reagent 2 3 After 3 4 minutes add 50 ul of Sample Serum 4 Read immediately note your readings 5 Normal Values 0 50 ALP Alkaline Phosphate 1 Take 400 ul of Reagent 1 2 Add 100 ul of Reagent 2 3 After 3 4 minutes add 10 ul of Sample Serum 4 Read immediately and note your readings 5 Normal Values 45 260 Total Proteins T P 1 Take 500 ul Reagent 2 Add 10 ul Sample Serum 3 Read After 5 minutes and note your readings 4 Normal Values 5 7 7 8 Serum Albumin 1 Take 500 ul Reagent 2 Add 5 ul Sample Serum 3 Wait for 5 minutes and note your readings 4 Normal Values 3 4 5 4 Serum Bilirubin 1 Take 100 ul Reagent 1 2 Add 25 ul Reagent 2 3 Add 500 ul Reagent 3 4 Now add 100 ul of Sample Serum in the mixture and wait for 10 minutes 5 After 10 minutes add Reagent 4 and wait for 5 minutes 6 After 5 minutes note your readings 7 Normal Values 0 4 1 1 Bilirubin Direct 1 Take 100 ul Reagent 1 2 Add 25 ul Reagent 2 3 Add 100 ul Sodium Saline NaCl 4 Add 100 ul Sample Serum and wait for 5 minutes and note your readings after 5 minutes 5 Normal Values 0 2 0 4 Bilirubin Indirect C Reactive Proteins CRP 1 By knowing T bill and Direct bill we can find out the Indirect Bilirubin 1 Take 2 ul Sample Serum 2 One drop of Reagent If separation shown then positive else negative R A Factor 1 Take 20 ul Sample Serum on slide 2 Add one drop of Reagent If separation shown then positive else negative ASO Titer 1 Same as CRP and R A Factor Prothrombin Time PT 1 Take 200 ul Reagent in the test tube 2 Set the temperature 3 Add 100 ul Sample Serum taken from PT voil 4 Note the clotting time on the stop watch Activated Prothrombin Time APTT 1 Take 200 ul Reagent of APTT in the test tube 2 Add 100 ul Sample Serum taken from PT voil 3 Set the temp To 50 C or Above 4 Add the 100 ul Calcium Note the clotting time Lipid Profile Complete Includes Cholesterol Triglycerides High Density Lipids HDL Low Density Lipids LDL Very Low Density Lipids VLDL Total Lipids Cholesterol 1 Take 500 ul Reagent 2 Add 5 ul Sample Serum 3 Wait for 5 minutes and note your readings 4 Normal Values 150 200 Triglycerides 1 Take 500 ul Reagent 2 Add 5 ul Sample Serum 3 Wait for five minutes and note your readings 4 Normal Values 50 150 It should be know that by knowing 2 values we can calculate also find the third 3rd value High Density Lipids HDL 1 Take 200 ul Reagent 2 Add 100 ul Sample Serum 3 Now Centrifuge the Solution and pick 5 ul from the solution and add to the 500 ul Reagent of cholesterol and wait for 5 minutes 4 After 5 minutes read the solution and note your readings 5 Normal Values 30 50 Low Density Lipids LDL 1 We can calculate the LDL by dividing or simply using formula E g Cholesterol HDL VLDL LDL 235 35 50 150 Very Low Density Lipids VLDL 1 We can calculate the VLDL by dividing Triglycerides by 5 E g 100 5 20 A Mathematically Distribution Suppose Cholesterol 200 Trig 150 HDL 40 LDL 200 40 50 110 VLDL 50 Total Lipids Cho Tg HDL 200 200 150 40 200 590 Screening Tests HCV Screening 1 Take 10 ul Sample Serum and put in the HCV kit Add 1 2 to drops of HCV buffer 2 If there will be 1 line show in the front of C the result will be Negative 3 If there will be 2 lines in the front of C and T the result will be Positive HbsAg Screening 1 Take 20 ul Sample Serum and 1 2 drops of buffer put in HbsAg kit same as HCV VDRL HIV Typhoid Mycodot H pylori Syphilis Aids IgG IgM Same as HbsAg Malaria Parasite MP Urine Pregnancy Test UPT 1 Take 20 ul EDTA blood and 1 2 to drops of buffer put into the M P kit note the result 1 Dip the stip in the urine for 7 10 seconds If there will be the 1 Line result will be Negative 2 If there will be 2 lines result will be Positive 3 Else negative Blood Grouping Test 1 Put three drops of EDTA blood on the grouping plate now add one one one drop of A B and O on the Widal Test 1 Before test Reagent O and H should be at moderate Temp Like 35 C 37 C 2 Now put the 20 20 ul Sample Serum on the slide seperatley Add O Reagent on one drop and H Reagent on other drop If there will separation Shown in O H the result will be Positive otherwise Negative TLC Total Lekocytes 1 Take 380 ul Reagent Add 20 ul EDTA blood After 10 minutes make slide blood drops Fig Fig 2 Count the number of while cells and multiply with 50 E g …