11 26 2013 Enzyme Specific type of catalyst that increases the rate of a chemical reaction by lowering activation energy catecholase potato extract Substrate A molecule that is acted upon by an enzyme catechol Four facts that can effect enzyme activity temperature pH amount of substrate source of enzyme When comparing solutions of different concentrations the one that has more solute is known as hypertonic and the one with less solute is known as hypotonic 7M KCl is hypertonic to 4M KCl Solvent is the liquid in which a solute dissolves Name the two factors that affect the ability of a molecule to cross the membrane Size and polarity Predict the hemolysis rate for mammalian RBC s caused by the following alcohols suppose they have same polarity 1 to 4 from longest to shortest time 2 pts Predicting hemolysis time of blood cells Lower the molecular weight faster hemolysis occurs Name A B C D Molecular Weight 120 50 30 60 You have 2 boxes A and B Suppose you have one potato cell in each box You fill Box A with a solution which is hypertonic more concentration to the concentration of the cell You fill Box B with a solution which is hypotonic less concentration to the concentration of the cell Predicted Hemolysis Longest 1 3 Shortest 4 2 A Indicate in the boxes the direction of net movement of water through the cell wall 2 pts Box A Out of the cell Box B Into the cell B What is this movement of water called 1 pt Osmosis The manufacturers of Rice Maker Fertilizer claim that their fertilizer will increase the productivity of rice grain if used as directed To test this a researcher applied the fertilizer to rice plants over the course of 6 weeks Productivity was determined by the mass tons of the grain produced Based on the statement above write appropriate null and alternative hypothesis 4 pts Null Applying Rice Maker Fertilizer to rice plants over 6 weeks will not increase plant productivity Alternative Applying Rice Maker fertilize to rice plants over 6 weeks will increase plant productivity Paper Chromatogrpahy separating mixtures of substances into their compounds Stationary phase the paper being used Mobile phase carries components up the paper Carotenoids run faster than chlorophylls because their particles are smaller Chlorophyll B is slightly more polar than Chlorophyll A Fermentation anaerobic process in which organic foods are converted into simpler compounds and ATP is produced Alcohol fermentation Lactic acid fermentation Gel electrophoresis separation and analysis of DNA based on size and charge Standard deviation measure of how spread out the data is from the mean Mean Average of data Affect of light intensity on the rate of photosynthesis as light intensity increases so will the rate of photosynthesis until a certain point Enzymes are generally proteins Spectrophotometer is an instrument designed to detect the amount of light energy being absorbed by molecules Example experiment from lab analyzing enzymes and getting the absorbance of benzoquinone Photosynthesis process by which light energy is converted into chemical energy that is used to produce organic compounds in plants Oxygen is generated and released as a by product of photosynthesis when WATER is split during the light reactions Micropipette Volumetric Pasteur pipettes Transfer pipettes 11 26 2013 11 26 2013