BISC 330L Biochemistry Spring 2015 USC Lecture Friday Jan 16 2015 1 Finish Chapter 1 material Henderson Hasselbalch Discovery of DNA The Genomic Revolution Henderson Hasselbalch Equation For acid AH proton donor in neutral form AH A H Ka A H AH logKa log H log A AH logKa log H log A AH logKa log A AH log H log H logKa log A AH pH pKa log A AH Henderson Hasselbalch Equation NOTE A AH 10 pH pKa A AH 10 pH pKa AH A 10 pH pKa Physiological buffer system 1mM in blood stream at pH 7 4 The Genomic Revolution Structure of DNA 1953 Bacteriophage X174 was the first genome to be sequenced a viral genome with only 5 368 bp Yeast S cerevisiae 1989 Human genome project 1990 2003 DNA sequence amino acid sequence protein structure protein function Genes 3x109bp Environment Epigenetics Behavior Diet Gene expression protein 3 of genome DNA is a physical entity with certain size and shape does its structure play a role in genome function 3 4 1 10 10meter Genome in 1D 2 meters Genome in 3D Cell nucleus 10 m 0 00001 meters Chromatin in 3D Explore the 3D structures of the human genome Tethered Conformation Capture TCC Kalhor et al Nature Biotechnology Jan 2012 Tethered Conformation Capture TCC Contact frequency matrix Contact frequency matrix Contact frequency map Inter chromosomal Contact frequency map Structure calculation from TCC data TCC data End of Lecture 3
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