Lab Practical 2 Review Guide Know the Sudan IV test Know what a positive negative reaction looks like Know what the test is testing for Know the Iodine Test Know what a positive negative reaction looks like Know what the test is testing for Know the Biuret Test Know what a positive negative reaction looks like Know what the test is testing for Know the Benedicts Test Know what a positive negative reaction looks like Know what the test is testing for Know the Dische Diphenylamine Test Know what a positive negative reaction looks like Know what the test is testing for Know the Grease Spot Test Know what a positive negative reaction looks like Know what the test is testing for Understand how Column Chromatography works Given a sample of tubes full of dye that has been separated by the column be able to identify which dye has the highest molecular weight the dye that comes out first Understand how Paper Chromatography works Know the stationary phase of paper chromatography Know the mobile phase of paper chromatography Be able to calculate Rf values and determine which amino acid has the greatest Rf value typically the amino acid that traveled the furthest on the paper Determine the contents of an unknown sample by comparing it to other known samples you did this in the homework Understand how Gel Electrophoresis works Determine what samples have a positive charge and what samples have a negative charge remember opposites attract Be able to place samples in order from smallest to largest remember smaller samples move further on the gel Determine the contents of an unknown sample by comparing it to other known samples you do this the same way as you would with paper chromatography Know the different parts of the plants we observed Know the difference between simple and compound leaves Know the different parts of the plant and their function fruit stem Be able to identify the different types of roots adventious fibrous root seed tap etc Be able to name the parts of the flower Sepal Stamen Pistil Stigma Anther Ovary Petal Know the parts of the peanut seed coat roots cotyledon etc Be able to identify the stomata under the microscope and know its function Understand gravitropism Understand the Microbiology Lab Be able to identify colony shapes under the microscope rod spherical helical Be able to determine colony morphology when not looking under the microscope curled rough round filamentous pigmented irregular Be able to determine which spices inhibited growth of a bacteria when looking at a sample of gel agars grown with different spices exactly like how you did in lab only less to look at Determine if a bacteria is susceptible or resistant to an antibiotic by measuring its zone of inhibition exactly like how you did in lab Be able to list 4 aseptic techniques be sure to know what aseptic means