Pre-lab Quiz Lab Exercise 5Pre-lab Quiz Lab Exercise 3 Part IIPre-lab Quiz Lab Exercise 4Pre-lab Quiz Lab Exercise 3 Part I Note: If you feel that there is more than one answer for any of the questions below, please feel free to CIRCLE ALL THAT APPLY! VERSION C 1. In today’s lab, what will be used to precipitate proteins using the ‘salting out’ method? a. Ammonium hydroxide b. Ammonium sulfate c. Potassium sulfate d. Ethanol 2. In today’s lab, you will attempt to precipitate protein from: a. Whole blood b. white blood cells c. Plasma d. Lysed Red blood cells 3. If a protein precipitates out of a solution containing a watery solvent, which of the following can be said about the agent that caused the precipitation? a. It must have been more polar than water. b. It reduced the solvation shell around the protein c. It increased the solubility of the protein. d. It may have been less polar than water. 4) Upon centrifugation, proteins that did not precipitate in a solution can be found: a. In a pellet b. Still dissolved in the watery solvent c. In the supernatant d. None of the above.KEYVERSION C 1. B (0.5 PTS)2. C (0.5 PTS)3. B, D (0.25 PTS EACH) 4. B,C (0.25 PTS EACH)Pre-lab Quiz Lab Exercise 5VERSION B1. The best definition of an agonist is a substance that:a. Inhibits a given cellular processb. Activates a given cellular processc. Has the opposite effect of a receptor’s natural ligandd. Mimics the effects of a receptor’s natural ligand2. The best definition of an antagonist is a substance that:a. Has the opposite effect of a receptor’s natural ligandb. Inhibits a given cellular processc. Activates a given cellular processd. Mimics the effects of a receptor’s natural ligand3. The average heart rate of the organisms you will be working with today is about:a. 300 beats per minute b. 75 beats per minutec. 1000 beats per minuted. 120 beats per mintute4. The sympathetic nervous system has the general effect of ______________ heart rate.a. Increasingb. decreasingPre-lab Quiz Lab Exercise 3 Part IINote: If you feel that there is more than one answer for any of the questions below, please feel free to circle all that apply!VERSION C1. Which of the following most accurately describes the critical role of SDS in the technique, SDS-PAGE?a. it unfolds the 3 dimensional structure of proteins b. it neutralizes the charge on proteinsc. it gives proteins an overall positive charged. it stabilizes the 3 dimensional structure of proteinse. it gives proteins an overall negative charge 2. Which of the following dyes will be used to track the movement of proteins during electrophoresis in today’s lab?a. Coomassie blueb. P-nitroanilinec. Trypan blued. Bromophenol blue e. Methylene blue3. The polyacrylamide component of a gel used for SDS-PAGE is used in order to:a. Separate proteins on the basis of charge and shapeb. Separate proteins on the basis of shapec. Separate proteins on the basis of size/length d. Minimize the differences in protein size/lengthe. None of the above4. Which of the following dyes will be used to visualize individual proteins after electrophoresis in today’s lab?a. Coomassie blueb. P-nitroanilinec. Trypan blued. Bromophenol bluee. Methylene bluePre-lab Quiz Lab Exercise 4 Note: If you feel that there is more than one answer for any of the questions below, please feel free to circle all that apply!Grading notes: 0.5 point each. VERSION C1. Enzymes function by:a. Lowering the activation energy of a given reaction b. Raising the activation energy of a given reactionc. Increasing the molecular motion of substrate reactantsd. None of the above2. At some point during today’s lab, you will be measuring/calculating:a. The absorbance of light at a fixed wavelength as a function of pNA concentrationb. The effect of temperature on the rate of product formationc. The amount of BAPA formed as a function of enzyme concentrationd. The rate of product formation as a function of substrate concentration 3. For a given enzymatic reaction that follows Michaelis Menten kinetics, the substrate concentration at which ½ of the maximum reaction velocity is reached is called:a. Vib. Km c. ½ Vmaxd. None of the above4. In general, the higher the affinity an enzyme has for its substrate, the _________ theKm value for the enzyme-catalyzed reaction.a. Lower b.
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