Unformatted text preview:

Biology 107 Spring 2021Lab Week 6 DiscussionTopic: Phages as ToolsQuestion 1: One application of phages is to quickly detect the presence of food-borne pathogenic bacteria in food. As an example, a microbiologist working for Chobani takes two batches of yogurt off of the production line. She suspects that one yogurt sample is contaminated with Listeria, a common food-borne pathogen, whilethe other one may or may not be contaminated. A. The scientist takes an extract from the contaminated yogurt sample and adds 1000 Listeria-specific virus particles. After shaking and incubation for a day, what will happen to the number of phage particlesin the sample? Why? Is this experiment selective for Listeria or could other bacteria lead to false positiveresults?There be more phage will reproduce B. Based on your answer to Part A., how could the scientist tell if the second yogurt sample was contaminated with either Listeria or with E. coli, another potentially pathogenic bacterial species?Less virus particles.Question 2: 2. Phage therapy is an alternative to antibiotic treatment for bacterial infections. In phage therapy, phages specific to pathogenic bacteria are delivered to the site of an infection (e.g. on wound dressings, by oral ingestion, through an IV). A. One side effect of antibiotic treatment is that these chemicals kill many beneficial bacteria in the human gut. Why might phage therapy, for example to combat a Listeria infection in the gut, be superior? Superior B. One problem with antibiotics is that they are typically unstable and quickly degrade in the body resulting in the need for frequent, high doses during treatment. What advantage would phage therapy have over antibiotic treatments in this respect?Only need one doze of phage because they have the ability to multiplyQuestion 3: A hypothetical situation: A bacteriophage discovered by a student in the SEA-PHAGES program shows very strong infectivity of its bacterial host (it kills them quickly in culture). The genome of this phage is in the Phages Database, and it has been annotated.Based on your growing understanding of comparing phage genomes, if you wanted to determine which other annotated phages that infect the same bacterial host might also kill this host effectively, but you could only test a small number of phages provided by the phage archives, what could you do to help you narrow down the pool of candidate phages to request for testing?Compare to other genes, Prosocial 5, Spring


View Full Document

WSU BIOLOGY 107 - Lab Week 6 Discussion Sp 21

Documents in this Course
Exam 1

Exam 1

3 pages

Load more
Download Lab Week 6 Discussion Sp 21
Our administrator received your request to download this document. We will send you the file to your email shortly.
Loading Unlocking...
Login

Join to view Lab Week 6 Discussion Sp 21 and access 3M+ class-specific study document.

or
We will never post anything without your permission.
Don't have an account?
Sign Up

Join to view Lab Week 6 Discussion Sp 21 2 2 and access 3M+ class-specific study document.

or

By creating an account you agree to our Privacy Policy and Terms Of Use

Already a member?