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Biology 107 Spring 2021Lab Week 4 Discussion(Week of Feb. 8)Topics: Journal Article AnalysisThe following questions are related to the paper from Huang et al. “Isolation, characterization, and application of a novel specific Salmonella bacteriophage in different food matrices” that you read for this week.Question 1: The researchers were looking for bacteriophage, which are abundant in most environments, but they collected environmental samples from some pretty foul (and fowl) locations… what were those locations and why look for phage there? Where else would you have looked for these phages?Public gyms, public restaurants restroomsQuestion 2: Here are 3 sentences from the manuscript. If you were writing a manuscript, which section (introduction, Materials and Methods, Results, Discussion) would you place each statement in? Justify your answer… for example what was the specific part of each statement that provided evidence of its origin?A. “Among the isolated phages, phages LPST10, LPST11, and LPST13 were isolated from sewage near a river, while phages LPST18, LPST21, and LPST23 were isolated from sewage of a wastewater treatment plant. Phage LPST10 could form a relatively larger and clear plaque with distinct edges with a diameter of0.5 mm in double-layer agar plate...” methodsB. “…many studies have shown that the resistance of Salmonella spp. to antibiotics may cause food safetyissues owing to potentially insufficient sanitation (Balk, 2011). In view of this condition, bacteriophages, which show distinguished resistance mechanisms, when compared with antibiotics (Torres-Barceló & Hochberg, 2016), have received renewed attention in recent years with regard to controlling foodborne pathogens.” introductionC. “… the findings of this study provide a solid basis for further research on phage LPST10 and its combination with other phages in phage-based biocontrol of Salmonella contamination in foods.” Conclusion/summary/discussionQuestion 3: What is the next set of experiments that the authors could/should do related to this project?Different phages /amounts Prosocial 3, Spring


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WSU BIOLOGY 107 - Lab Week 4 Discussion Sp 21

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