Post Lab AssignmentBacterial transformation conceptsTransformation experimentTransformation EfficiencyName:ONLINE Transformation Post-Lab AssignmentPost Lab AssignmentStudents will be expected to complete this assignment after reading/watching the associated contentfor this assignment in the Online Week 1 folder on eLC. This post-lab assignment will require you to understand the concepts and importance of genetic transformation, gene regulation, and the techniques/pathways through which they are achieved.This assignment should be completed individually in adherence to the UGA Academic Honesty Policy.Use of sources outside of material posted in eLC or the lab manual is not all owed . All responses should convey student understanding of the concepts and content presented and represent their original thought and written work.Please submit your PDF to the eLC drop box by 9:00 P.M. Sunday April 5 2020.Bacterial transformation concepts1. In your own words, what is genetic transformation? (1 pt)Genetic transformation is a process in which DNA of an organism is changed by inserting a foreign gene into the cell.2. How is genetic transformation beneficial to an organism in its natural environment? (1 pt)Genetic transformation helps to make a modified version of the organism which is more adaptive to its surrounding.3. What is a plasmid? (1 pt)Plasmid is a circular DNA which is present in bacteria.4. What characteristics make plasmids ideal for performing transformations? (1 pt)Plasmids are very small, thus it can carry the gene easily from one organism to another. Plasmids have multiple binding sites which means the plasmid can be cut and a section of a foreign gene can be pasted on the binding site.5. Why is bacteria an ideal model organism for transformation experiments? (1 pt)Bacteria is an ideal model organism for transformation because only bacteria have plasmids and ribosomes which is ideal for DNA replication and production.ONLINE Transformation Post-Lab AssignmentTransformation experiment1.What feature of the pGLO plasmid’s expression will identify a successful transformation? (1 pt)pGLO act as gene marker. It is initially inserted into the plasmid to observe the transformation process. If the end product of the sample shows a color by shining on fluorescent light, then it can be confirmed that it was a successful transformation.2. What bacterial growth pattern would you expect on each of the four agar plates above and why?(4 pts)A)less pattern will be seen than others because it does not have arabinose.B)More growth pattern will be seen than others because it has arabinose than increases productionof pGLO.C)No pattern will be seen as there is no gene marker.D)No pattern will be seen as there is no amp/ara and also no gene markers.3. Which agar plate(s) serve as your positive control? How do(es) the plate(s) serve this role? (3 pts)B serves as positive control. This is because4.Which agar plate(s) serve as your negative control? How do(es) the plate(s) serve this role? (3 pts)D serves as negative control. This is because it does not have amp or ara as well as no pGLO, thus itcan be compared with others.ONLINE Transformation Post-Lab AssignmentTransformation EfficiencyUse the following equation and sample plate to determine the sample efficiency of the trial.𝑇𝑜𝑡𝑎𝑙 # 𝑜𝑓 𝑐𝑒𝑙𝑙𝑠 𝑔𝑟𝑜𝑤𝑖𝑛𝑔 𝑜𝑛 𝑡ℎ𝑒 𝑎𝑔𝑎𝑟 𝑝𝑙𝑎𝑡𝑒𝑇𝑟𝑎𝑛𝑠𝑓𝑜𝑟𝑚𝑎𝑡𝑖𝑜𝑛 𝑒𝑓𝑓𝑖𝑐𝑖𝑒𝑛𝑐𝑦 =𝐴𝑚𝑜𝑢𝑛𝑡 𝑜𝑓 𝐷𝑁𝐴 𝑠𝑝𝑟𝑒𝑎𝑑 𝑜𝑛 𝑡ℎ𝑒 𝑎𝑔𝑎𝑟 𝑝𝑙𝑎𝑡𝑒 𝑖𝑛 𝜇g To find the number of cells growing you must count the number of glowing colonies present on theLB/amp/ara plate shown below.The amount of DNA spread on the plate refers to the total amount of pGLO plasmid introduced duringthe experiment. Assume that the experiment was done according to the posted Transformation Protocol. (Hint: 10µl of the DNA plasmid was used and the plasmid was provided at a concentration of0.08 µg/µl.)Calculated transformation efficiency (1 pt):Would you consider this to be a successful experiment? Why or why not? (3 pts)Yes I will consider this as a successful experiment because Transformation efficiency is greater than