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UNC-Chapel Hill BIOC 108 - Lab 03 Notes

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Lab 03 Notes:Structure of Circulating Serum Lipoproteins: » Fats are nonpolar, so how do we move them around as our most efficient storage form? » Answer: Serum LIPOPROTEINS Hydrophilic outer layer = apolipoproteins & phospholipids Hydrophobic inner core = triglycerides and cholesterol ester Cholesterol keeps the inside fluid Apolipoproteins deliver triglycerides, allows contents to beemptied into adipocytes for storageSize and Density of Lipoproteins: Chylomicrons are the largest lipoproteins, made in the gut Adipocytes quickly take them up All cells need cholesterol to keep core fluid/liquido LDL (lipoprotein) is a delivery vehicle to give cholesterol to other cells  Can cause clot in artery if excess is deliveredo HDL (lipoprotein) picks up unneeded cholesterol Cholesterol Assay: measuring all cholesterol in an individual’s blood First, we covert cholesterol esters to cholesterol and a fatty acid by hydrolyzing them  Next, oxidize this free cholesterol to form H2O2 (hydrogen peroxide) Convert hydrogen peroxide to a colored


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