Micrometer Worksheet- Spring 2019 – MUST BE TYPED*YOU WILL LOSE 5 POINTS ON THIS WORKSHEET IF YOU BREAK A STAGE MICROMETER Show all calculations Use the following equation to answer the micrometer questions (page 156 of the lab manual also has thisequation):1 ocular space = distance on the stage micrometer in µm OR number of spaces on stage micrometer x 10µm number of spaces on the ocular micrometer number of spaces on the ocular micrometer  On our stage micrometers, the small (minor) lines are 0.01mm (10µm) each and the large (major) lines are 0.1mm (100µm) each.1. If 18 ocular divisions line up with two minor divisions of the stage micrometer, what is the diameter of afungal cell that spans 3 ocular divisions? (6pts)If two stage divisions equal 20 um which equal 18 ocular divisions, then each ocular division equals 1.11 um (20 um/18 ocular divisions). A fungal cell that spans 3 ocular divisions would be (3 x 1.11 um) 3.3 um (micrometers) in diameter.2. A human RBC is approximately 7 µm in diameter. An unknown bacterium measures 1 ocular space using the oil immersion objective compared to 2 minor spaces on the stage micrometer. A) How big is the unknown bacterium (report in micrometers) and B) how does its size compare to the RBC (how many times bigger is one versus the other)? (6pts)Size= [# of minor spaces on stage micrometer x10 um (micrometers)]/ [# of ocular spaces]Size= [3 spaces x 10 um (micrometers)]/1 = 30 um (micrometers)The bacterium is between 4 and 5 times bigger than the human RBC. The exact amount of times larger is 4.29.3. When increasing magnification, what will happen to the number of spaces on the stage micrometer compared to the spaces on the ocular micrometer? (2pt)The number of spaces on the stage micrometer will decrease because the size of the ocular division graduations will also increase.4. You have some bacterial samples which you need to characterize and need to measure the bacteria. Below is a view similar to what you see through your microscope as you calibrate it. Also shown are thebacteria you need to measure. Calibrate your ocular micrometer and give the length of bacterium A and width of bacterium B in m. Show your work. (6pts)The image shows that the lines show perfect alignment at the 6th unit of the stage micrometer (60 um (micrometer)) and the 10th unit of the ocular micrometer. To calibrate the ocular micrometer atthis objective, the following equation was used.(1 x 60 um) = 60 um = 6 um (micrometers) = 6 um (micrometers) per ocular unit10 ocular units 10 ocular units 1 ocular unitAfter calibration, the following measurements of bacteria were obtained.Length of bacterium A= [15 (spaces on ocular micrometer)] x [6 um (micrometers) per ocular unit]= 90 um (micrometers) longWidth of bacterium B= [50 ocular units – 47 ocular units] = 3 ocular units[3 (spaces on the ocular micrometer) x [6 um (micrometers) per ocular unit] = 18 um (micrometers)