Ashlee FaloniBio 001Lab 3SECTION I – Health and SafetyNO QUESTIONS, but you will need to hear this safety induction in order to proceed in the lab.SECTION II – MeasurementsQ1) How many micrometers are there in a centimeter? 1000 nm/ 10^3 nm Q2) How many nanometers are there in a meter? 1000000000/10^8 nmActivity A.1 – LengthQ3) Which object did you select? The object we selected was rope. __________Q4 ) Estimate its length in centimeters. Estimate:_____12______ cmQ5 ) Measure its length using a ruler. Measured length:___12.5______ cmQ6) How close were you to the actual length? _____50________ mmComplete Table A.1 below with respect to the length of the same piece of string that youmeasured above:Table A.1: Interconversion between unitsACTIVITY IIAMeasured length of string = 12.5 cmLength in meters = 0.125mLength in kilometers = 0.000125kmLength in millimeters = 1250 mmLength in micrometers = 125000 μmLength in nanometers = 1.25108 nmPage 2 of 9Adapted from Spring 2016 version K. DulaiActivity A.2 – Weight is a measure of the mass of an object under gravitational pull.Q7) Which object(s) did you select and how much does it weigh? The teacher didn’t make us do this part. Activity A.3 – Volume is a measure of the amount of material.Q8) What is the weight of the water?The teacher didn’t make us do this part.Q9) Since one ml of water weighs one gram, what was the volume of water in yourmeasuring cylinder? The teacher didn’t make us do this part.Q10) How much did you select using the pipette? _________990mm______________ Q11) How accurate was the instrument? Within _____1___ μlSECTION III – Compound Light MicroscopyActivity IVA.1 Safe handling of the microscope.NO QUESTIONSACTIVITY IVA – Use of a microscopePage 3 of 9Adapted from Spring 2016 version K. DulaiActivity IVA.2 Examine your microscope and identify and label the following parts on Figure 1:Activity IVA.3 Observations with the compound light microscope.Observation 1. Calculating total magnification.Q1) How is the total magnification calculated? Magnification of object x ocular= total magnification is how the total magnification calculated. Q2) What is the magnification of your microscope in its current configuration?40 Magnification= 4x/0.10 is what the microscope in its current configuration. Observation 2. Depth of FieldQ3). Which of the cross fibers is on top and how do you know this? The fiber crossed on top is the red one and I know this because the red tone is more dominant. Observation 3. Specimen orientationQ4) Diagram what you see through the eyepiece at each of the following totalmagnifications: Tests your drawing skill!Observation 4. Physical properties of the microscope.As you increase the magnification in the step above, what happened with respect to the following properties?Q5) Diameter of the field of view: The diameter goes smaller. Q6) Brightness: The brightness decreases. Observation 5. Compare the orientation of the letter ‘e’ on the actual slide with its appearance under magnification.Q7) How is the viewed image orientated compared to the actual specimen? More than one change to note!The viewed image oriented compared to the actual specimen is it turns upside down, and the grooves on the side is shown as a boxy outline. Page 5 of 9Adapted from Spring 2016 version K. DulaiObservation 6. Use the stage controls to move the stage of the microscope.Q8a) If you physically move the slide on the stage left, which direction does the imagemove as viewed through the ocular lens? If you move the slide on the stage left, the image mores left as viewed through the ocular lens. Q8b) Which knob permits you to move the stage up and down towards or away from theobjective lens? Coarse adjustment knob is the knob you move that moves stage up and down towards or away from the objective lens. Activity IV A.4 Determining actual size using a microscope Table A.4: Calibrating ocular micrometer.Total Magnification Size of one measurement unit (mm/unit)40x (use size calculated from grid slide)30 mm100x32 mm400x 8 mmActivity IVA.5 Live Specimens – Wet MountsQ9) Draw several cells in the space below, annotating the cellular parts that you see.Activity IV A.6 Measuring the length of several Elodea cellsHaving previously calibrated your ocular micrometer, you may now use it to measure the lengths of cells in your Elodea sample. Remember to note which objective you are using (10x and 40x objectives will work best). Measure the length of four different cells on your wet mount slide. Then, determine the average length of an Elodea cell by calculating the mean of the four cell lengths you measured.Q10)_____4____ mm, ___4_____ mm, ____5____ mm, _____5___ mm Average =____4.5____ mmQ11) Why is it necessary to take more than one measurement on the size of the Elodeacells?It is necessary to take more than one measurement on the size of the Elodeacells because they are not the same size. Activity IV.7 – Enhancement by StainingQ13) Was the slide stained with methylene blue solution better for viewing and seeing structures than the unstained slide? Why?It is necessary to be stained with methylene blue solution because it is easier to see theindividual parts. Activity IVA.8 Make several wet mount slides of pond water and explore life in a drop ofwater. Your instructor will demonstrate how to use slides and coverslips to make wet mounts. Try to identify as many organisms as possible using the illustrations/resources provided.Q14) Draw the organisms you found in the pond water. For each write down the feature that caught your interest! (NOTE: do NOT write vague statements such as "it looks pretty cool". Try to write something more definitive about the structures you actually observe, their movements, colors,