Clemson BIOL 1101 - Lab 4 Topic 2: Microscopes and Cells (11 pages)

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LAB TOPIC 2 1e can Cells study uted nee cal sts J Mi crosco12 es and Cells 5 b 10 l lilv tj ese light and n in a1ose h is in OiJ r a 0 1e ghere OWV f v v V t OV Y l f N f r A t Q V S AA y V t fuo t S too o e r peci FIGURE 2 1a The compound binocular light microscope Locate the parts of your microscope described in Exercise 2 1 and label this ph otograph Indicate in the margin of your lab manual any features unique to your microscope cular enses 1pe of e of tru re all le FIGURE 2 1b Enlarged photo of compound light microscope as viewed from under the stage Th is microscope is l uq ipped with phase contrast optics Locate the condenser condenser adj ustment knob phase contrast revolving turret and iris diaphragm on your microscope if present and label them on the diagram J LAB TOPIC 2 k For binocular m icroscopes cover your left eye and use the fine adjust Uf epiece time you letter ment knob to focus t he fixed righ t ocular until the letter e is in maximum focus Now cover t he rig ht eye and using the diopter ring on the left ocular bring the Image into focus The letter e should now be in focus for both of your eyes Each new lab day as you begin to study you r first slide repeat this procedure I You can increase or decrease the co ntrast by adjusting the iris diaphragm opening Note that the maximum amount of ligh t provides little con trast Adjust the aperture until the image is sharp t m Move the slide slowly to t he righ t In what direction does the image in er n t he ocular move n Is the image in the ocular inverted relative to the specimen on the stage yslowly he ion the tr lab cale he S il the he both o Center the specimen in t h e field of view th en rotate the 40X ob jective in to position wh ile watching from th e side If it appears that the objective will hit the slide stop and ask for assistance Most of the microscopes have parfocal lenses which means t hat little refocusing is requ ired w hen moving fro m one lens to another If your scope is not parfocal