UGA POUL 3000L - Written Lab Study Guide 3000 (7 pages)

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Written Lab Study Guide 3000



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Written Lab Study Guide 3000

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Pages:
7
School:
University of Georgia
Course:
Poul 3000l - Avian Biomed Tech
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WRITTEN LAB EXAM POUL 3000 Gross Anatomy 1 Know the following directional terms a Dorsal b Ventral c Medial d Cranial e Caudal f Proximal g Distal 2 Know the following bones from the picture of skeleton of the fowl in the gross anatomy handout a b c d e Incisive Mandible Occipital Atlas Clavicle f g h i j k l m n o p q r s t u Humerus Radius Ulna Scapula Metacarpus Ilium Ischium Femur Sternum Coracoid Tibia Fibula Metatarsus Uncinated process Sternal or vertebral rib Pubis 3 Be familiar with the female reproductive tract photo in the gross anatomy handout a Infundibulum b Magnum c Isthmus d Shell gland e Vagina f Cloaca Hematology Lab 1 What was the difference between the heparinzed tube and non heparinzed tube Heparinized contains heparin an anticoagulant to prevent clotting 2 What is the typical PCV for a chicken 35 9 41 0 a Would it be higher in males of females Higher in males testosterone stimulates erythropoietin which increases erythropoiesis to production of RBC Females have yolk precursors which take a percentage out of blood components to blood 3 Be able to name all leukocytes and their general role in and appearance in chicken Heterophils WBC granulocyte leukocyte bacteria Eosinophil WBC granulocyte leukocyte parasites Basophil WBC granulocyte leukocyte B lymphocyte vs T lymphocyte WBC Agranulocyte leukocyte Monocyte Macrophage WBC Agranulocyte leukocyte 4 What is H E stain a What does hematoxylin stain and what color Dark blue or violet Basic positive that stains basophilic substances acidic and negatively charged DNA RNA b Eosin Is red pink acidic negative stain binds to acidophilic substances positively charged A A s lysine and arginine 5 You may be given a screen shot of four different hemocytometer squares using blood that has been diluted 1 10 in saline You will need to determine the red blood cells per ml in the original sample in case you forgot how http www abcam com protocols counting cells using a haemocytometer Total of 4 squares 4 X 10 000 X 10 cells mL Antimicrobial Laboratory 1 What are some differences between gram positive and gram negative bacteria Gram stains purple and thick peptidoglycan Gram stains pink and thin peptidoglycan layer with outer membrane endotoxins and exotoxins 2 Be familiar with the differences and selectivity of the following media growth plates a LB Lysogeny broth General growth plate b MSA mannitol salt agar gram bacteria bc high salt concentrationstaphylococcus aureus yellow zones staph epidermis white c EMB eosin methylene blue coliforms E coli gram bacteria lactose presence lactose fermentation 3 You may be give a screen shot of an agar diffusion test that needs to be interpreted Zone of inhibition how effective abx is at stopping growth Introduction to Surgery and Anesthesiology 1 What is the carrier gas that we used to deliver the isoflurane ins a vaporized form O2 2 At what flow rate is the vapor delivered to the bird 1 5L min 3 Into where is the intubation tube inserted Glottis 4 At what level is isoflurane used for induction of anesthesia 5 5 At what level is it maintained during surgery 1 5 2 5 6 Know the general characteristics of anesthesia Inhaled or Injectable Inhaled better bc can be titrated more consistent therapeutic index rapid induction recovery minimal cardioresp side effects 7 What methods are used to monitor the bird for proper levels of anesthesia Pinch toes check reflexes monitor HR for increases movement 8 Be able to provide the procedure for preparing the surgical site prior to the incision Select target area of incision pluck feathers move legs out of the way with cord wipe with betadine then ethanol 2 times cover with sterile drape inject lidocaine SQ 9 What is lidocaine and how does it work Lidocaine is local anesthetic Inhibits VG channels Na blocks nerve impulses 10 What is the advantages of choosing either simple interrupted over simple continuous Simple interrupted is more secure closure bc if one suture breaks the remaining will hold less infection bc microorganism less likely to travel along sutures Simple continuous faster snug 11 Why is blunt dissection a better method than cutting for the displacement of connective tissue at the surgical site 12 Why is it important to lower the head so that the birds mouth faces the table Below From Suture Lecture 13 Know the size range for sutures a 10 0 smallest to 7 largest b Why should size be taken into consideration when choosing the proper suture size 14 What is property of elasticity important to consider when choosing suture size 15 Advantage disadvantage of having a high amount of plasticity in the suture 16 Characteristics of suture material important for ease of handling and know security a What would be the advantage disadvantage of a suture with a low coefficient of friction b What would be the advantage disadvantage of a suture with a high memory c Advantage Disadvantage of monofilament vs multifilament i Which of the above should be used on skin or exposed wounds d Advantage disadvantage of absorbable vs nonabsorbable 17 You may be presented a screen shot of a suture package you will need to tell me the following from the package a Needle shape needle point type it will be either taper cut or cutting edge suture size gauge suture material 18 We often used 3 0 or 4 0 polyglycolic or chromatic catgut and 3 0 black silk be familiar with when each were used and why 19 What are the roles of Amikacin Enolfloxin and meloxicam 20 Be able to describe how a Lone star retractor assists with surgery 21 Be able to provide a description of the recovery process starting with the suturing of the skin and ending with placement of the bird in its recovery room 22 Why is the heating pad used during surery 23 Why is it important to ensure proper placement of the dispersion pad 24 How does the electrosurgical unit VetStar work 25 Be familiar with the different VetStar settings and how they differ from each other Follicle removal 1 Through which side and where was the initial incision made for this surgery 2 What is peritonitis and why was it a risk for this surgery 3 How is the follicle attached to the ovary and what was used to assist with retraction of the follicle 4 Which follicle was removed Egg removal 1 From what portion of the oviduct was the egg retracted 2 Why was it important to ensure the placement of serosa and mucosa when closing the oviduct 3 Where was the incision made for this surgery a What precaution is needed when addressing this surgical


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