Biochemistry 694301 Second Exam, Dr. Deis Mon. Aug. 1 1,2008 Name L. _,. - . . Row Letter - Seat Number - This exam consists of two parts. Part I is multiple choice. Each of the 25 questions is worth two points. Answer Part I questions on this sheet, below. Answer the Part I1 questions on the question pages. Please use BLOCK CAPITAL letters like this --- A, B, C, D, E. Not lowercase! 1. 10. A 18. c. GRADE: Part I Total q-6 Part 11: 11- 1 11-2 11-3 I1 -4 11-5 Part I1 Total .3$. < I did not cheat on this exam. SIC- n.< Total, I & I1 https://www.coursehero.com/file/6195944/Second-Exam-Aug-08/This study resource wasshared via CourseHero.com694:301 Second Exam, page 2 Name L assumption can we make about the energetics of simple osmosis? is zero, and K, = 1.0 D. Low K,,, means tight binding E. Osmotic pressure = nRT C. AGOy is positive 2. Which of the following is an "ABC transporter"? A. Calcium pump D. Acetylcholine receptor @ - ~ultidru~ res. protein E. GLUT4 receptor C. Voltage gated K+ channel 3. Symport and Antiport are two examples of what? ,-K.' simple diffusion ,Wprimary active tra>sport @$ facilitated diffusion 6none of the above C. secondary active transport 4. Protein Kinase A causes phosphorylation of the target sequence RRGSE in its substrates. - The Regulatory Subunit has the sequence A. ARGSE @. RRGAE B. RAGSE E. RRGSA C. RRASE 5. The second messenger for the system that activates ProteinIenase A is A. Calcium D. cyclic GMP B. 13 BPG (&cyclic AMP C. DAG \--' 6. Protein Kinase C activation is triggered by G-protein activation of a membrane bound enzyme, Phospholipase : A. A D. D &: E. all of the above 7. In the "fluid mosaic model" the mortar of the mosaic is lipid, and the stones are A. cholesterol D. oligosaccharides @roteins E. Calcium sulfate crystals &RNA 8. What is generally true about glycoproteins, proteins with sugars attached? A. found in cytoplasm @$transmembrane proteins have sugars on OUTSIDE surface *C: transmembrane proteins have sugars on INSIDE surface D. transmembrane proteins have sugars on BOTH surfaces E. none of the above 9.* Which membrane lipid is represented below? A. 'sphingosine D. Sphingomyelin OH weramide I E. a Ganglioside - = c ,c, c - c - oIJ C: a Cerebroside ) cN - +a https://www.coursehero.com/file/6195944/Second-Exam-Aug-08/This study resource wasshared via CourseHero.com694:301 Second Exam, page 3 Name - 10. The 4epimerase which changes Galactose into Glucos_e allowing it to enter Glycolysis acts on which substrate? @ UDP Galactose D. Galactitol B. Gal-I-P E. Gal-6-P C. plain Galactose 11. Which enzyme of Glycolysis has a large positive standard free energy change. In other words it would "much rather go backward"? A. Hexokinase D. PFK-1 Q~ldolase E. Glucokinase C. G3PDH 12. The mechanism of Aldolase begins with a covalent reaction between carbon two of F- 1,6-BP and a residue of which amino acid in the enzyme's active site? A. ser D. his B. cys QY~ E. glu 13. What is the value of Atkinson's Energy Charge Function when [AMP] = 5 M, [ADPI= 2 M, and [ATP] = 3 M? A. 0.25 D. 0.5 '7 -3 + iL Ll -/ B. 0.33 E. 0.65 - - D0.4 F. 0.9 T+ T42 I0 14. The complete process of gluconeogenesis resulting in production of Glucose occurs - - mainly where in the human bodg A. brain B. adipose C. salivary gland 15. Oxidation of which compound will produce the least energy? A. formaldehyde D. methane E. formic acid. ~a~~~~ oxide 16. Using the "El, E2, E3" terminology for _ Pyruvate-DH _-- -- Complex - Lipoamide is covalently attached to which enzyme? AC, (,I can Y--- A. El c. I33 C6~k ,,7Pr \ 0 E2 I D. none of the above : 0 , i; ,,,' "^.A priobrr \ \ TAD c t'? f r'-, 17. And Dihydrolipoyl Transacetylase is also known as rian7 A. El C. E3 ?9~2 D. none of the above https://www.coursehero.com/file/6195944/Second-Exam-Aug-08/This study resource wasshared via CourseHero.com694:301 Second Exam, page 4 Name L - - - -. - - - . 18. What cofactor would be most likely to be used for the reduction of a carbon carbon double bond CH=CH -> CH, - CH,? A. FADH, @~.tucc *=-P [!zEH - 19. The RQ or respiratory quotient is the --- Vmax -- of oxvgen use divided by the resting rate. whatisthe RQ for normally coupled mitochondria? (Note, the RQ for uncoupled mitochondria is 1) \i ,%^a -f o= A. 1 @ 10 / B. 2.5 E. 12.5 -4 u-cj f e5-\\'> 3 C. 7.5 20. The compound Atractyloside inhibits A. Complex I D. Uncoupling B. Complex 111 ~DP-ATP Translocase C. Complex IV kGlycer01-P Shuttle 21. In the mitochondria1 electron transport chain, cytochrome c, is reduced directly by mc D. a E. a, F. none of the above 22. In brain and skeletal muscle, complete metabolism of a molecule of Glucose yields how many ATP? = =% B3q C. 32 I?% 23. The Citric Acid Cycle occurs where in eukaryotic cells? A. the cytoplasm D. the mito. intermembrane space B. the lumen of the E.R. qthe mitochondria1 matrix C. the nucleus 24. Which statement is incorrect? Succinate Dehydrogenase ... A. has iron-sulfur clusters ~talyzes an oxidative decarboxylation @ contains FAD Ex11 of the above C. contains integral membrane proteins 25. If Homer Simpson had actually eaten the toxic part of the Fugu fish, what would be his first symptom of poisoning? A. heart explodes 6.1 lips feel numb B. a red rash E. upset stomach C. pain in the joints https://www.coursehero.com/file/6195944/Second-Exam-Aug-08/This study resource wasshared via CourseHero.com694:301 Second Exam, page 5 Name - --- --- PART I1 Answer these questions here on the question pages. 1. a. Draw a steroid nucleus, and dk Lecithin (phosphatidyl choline) - [ -0- c (3) ) \ / 0 - C - ,A' I \ H3 b.* Label each lipid "prokaryotic", "eukaryotic" or "archaeal" c. Sketch the Acetylcholine Receptor, an occur when Acetylcholine amves at the postsy%pxmmbme~Wh~e main difference between how the ACH Receptor allows sodihm into the neuron and how the Sodium channels found away from the synaptic cleft on the neuron membrane do the same job? 1 I I https://www.coursehero.com/file/6195944/Second-Exam-Aug-08/This study resource wasshared via CourseHero.com'?.'p~ 3SVNDI NIXLO~CI / 30 uo!)e~!~r? aq, n! paqoAu! ale saln3aIour asayl jo q~o~ '11a3 aq) ur saop I! JeqM Ira) a3ua)uas auo u! nay) pne aweu Lq Ljguap! '~o~aq u~oys a~n)~nr)s y3va rod - B '2 https://www.coursehero.com/file/6195944/Second-Exam-Aug-08/This study resource wasshared via CourseHero.com694:301 Second Exam, page 7 Name - 1.. - / 3. a. Show Glycolysis starting with Fructose-l,6-Bisphosphate and